AIF1
GeneName
AIF1
Summary
AIF1, also known as Iba-1 or AIF-1, is a 17 kDa protein primarily expressed in microglial cells and plays a significant role in the immune response of the central nervous system. It is localised in various cellular components, including the cytoplasm, nucleus, and actin filaments, and is involved in actin filament dynamics and cell projections such as lamellipodia and ruffles. AIF1 functions in processes like phagocytosis and the inflammatory response, and it can bind to calcium ions, which may influence its activity in cellular signalling and migration.
Importance
AIF1 is relevant to: - Microglial activation and neuroinflammation, making it a potential target for understanding neurodegenerative diseases. - Regulation of immune responses, particularly in the context of inflammatory diseases and tissue repair. - Actin dynamics and cellular motility, which are crucial in various physiological and pathological processes. - The modulation of smooth muscle cell behaviour, relevant to vascular biology and associated disorders.
Top Products
For researchers investigating AIF1, we recommend two excellent primary antibodies that cater to various applications. The first is the highly regarded polyclonal antibody, Anti-Iba1 antibody - Microglia marker (ab5076), which has garnered an impressive 1747 citations, underscoring its reliability in immunohistochemistry (IHC) and western blotting (WB). This antibody is a trusted choice for those studying microglial markers. In addition, we offer the recombinant antibody, Anti-Iba1 antibody [EPR16588] - Microglia marker (ab178846). This product has been validated in multiple applications, including IHC, immunocytochemistry (ICC), western blotting (WB), and flow cytometry (FC), making it a versatile option for researchers seeking consistent performance across experiments. With 794 citations, it is well-established in the research community, providing confidence in its use for AIF1 studies.
Abcam Product Citation Summary
The data indicates a significant focus on the role of AIF1 in various contexts related to microglial activation and neuroinflammation, particularly in studies involving both rat and mouse models. The use of Abcam antibodies in Western blotting and immunohistochemistry highlights the importance of AIF1 in understanding neurological responses to different stimuli, including infections, injuries, and inflammatory conditions.
Abcam Product Citation Table
Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Post-translational modifications
Phosphorylated on serine residues.
Tissue Specificity
Detected in T-lymphocytes and peripheral blood mononuclear cells.
Cellular localization
- Cytoplasm
- Cytoskeleton
- Cell projection
- Ruffle membrane
- Peripheral membrane protein
- Cytoplasmic side
- Cell projection
- Phagocytic cup
- Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
Alternative names
G1, IBA1, AIF1, Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1, iba1, iba-1, iba 1