Key features and details
- Rabbit polyclonal to TARBP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab129519 was affinity purified using an epitope specific to TARBP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab129519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 182 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
功能Probable S-adenosyl-L-methionine-dependent methyltransferase which methylates RNA molecules such as tRNAs. In case of infection by HIV-1, it binds to the loop region of TAR RNA, a region also bound by RNA polymerase II. Binding of TARBP1 and RNA polymerase II to HIV-1 TAR RNA is mutually exclusive, suggesting that TARBP1 may function alone or in conjunction with HIV-1 Tat to disengage RNA polymerase II from HIV-1 TAR RNA. May act by methylating HIV-1 TAR RNA.
序列相似性Belongs to the RNA methyltransferase TrmH family.
- Information by UniProt
- FLJ30482 antibody
- Probable methyltransferase TARBP1 antibody
- TAR (HIV) RNA binding protein 1 antibody
All lanes : Anti-TARBP1 antibody (ab129519) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 182 kDa
Exposure time: 3 minutes
Detection of TARBP1 by Western blot of immunoprecipitate
ab129519, at 6 µg/ml, immunoprecipitating TARBP1 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
ab129519 was used at 1 µg/ml for blotting immunoprecipitated TARBP1.
Detection: Chemiluminescence with exposure time of 30 seconds.