Anti-Talin 2抗体[68E7] (ab105458)
Key features and details
- Mouse monoclonal [68E7] to Talin 2
- Suitable for: WB, ELISA, ICC/IF, IHC-Fr, IP
- Reacts with: Mouse
- Isotype: IgG2b
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Talin 2抗体[68E7]
参阅全部 Talin 2 一抗 -
描述
小鼠单克隆抗体[68E7] to Talin 2 -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, ICC/IF, IHC-Fr, IPmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment, corresponding to amino acids 489-911 of Mouse Talin 2.
-
阳性对照
- Fibroblasts
-
常规说明
Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37°C + 5%CO2The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
68E7 -
骨髓瘤
NS0/1 -
同种型
IgG2b -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105458于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
Use at an assay dependent concentration.
|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
靶标
-
功能
As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity. -
序列相似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
细胞定位
Cell junction > focal adhesion. Cell junction > synapse. Cell membrane. Cytoplasm > cytoskeleton. Focal adhesion plaques and synapses. - Information by UniProt
-
数据库链接
- Entrez Gene: 70549 Mouse
- SwissProt: Q71LX4 Mouse
- Unigene: 33645 Mouse
-
别名
- DKFZp451B1011 antibody
- DKFZp686I0976 antibody
- DKFZp686K0979 antibody
see all
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab105458 被引用在 9 文献中.
- Hadjisavva R et al. Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition. Cell Rep 40:111091 (2022). PubMed: 35858563
- Han SJ et al. Pre-complexation of talin and vinculin without tension is required for efficient nascent adhesion maturation. Elife 10:N/A (2021). PubMed: 33783351
- Kanoldt V et al. Metavinculin modulates force transduction in cell adhesion sites. Nat Commun 11:6403 (2020). PubMed: 33335089
- Böttcher RT et al. Kindlin-2 recruits paxillin and Arp2/3 to promote membrane protrusions during initial cell spreading. J Cell Biol 216:3785-3798 (2017). PubMed: 28912124
- Theodosiou M et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. Elife 5:e10130 (2016). WB ; Mouse . PubMed: 26821125
- Bouchet BP et al. Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions. Elife 5:N/A (2016). WB . PubMed: 27410476
- Fang KP et al. Both Talin-1 and Talin-2 correlate with malignancy potential of the human hepatocellular carcinoma MHCC-97 L cell. BMC Cancer 16:45 (2016). PubMed: 26822056
- Austen K et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol 17:1597-606 (2015). PubMed: 26523364
- Debrand E et al. Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms. FEBS J 276:1610-28 (2009). PubMed: 19220457