Anti-Talin 1 and 2抗体[8D4] (ab11188)
Key features and details
- Mouse monoclonal [8D4] to Talin 1 and 2
- Suitable for: IP, Flow Cyt, ICC/IF, WB
- Reacts with: Rat, Chicken, Human, Xenopus laevis, Chinese hamster
- Isotype: IgG1
概述
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产品名称
Anti-Talin 1 and 2抗体[8D4] -
描述
小鼠单克隆抗体[8D4] to Talin 1 and 2 -
宿主
Mouse -
经测试应用
适用于: IP, Flow Cyt, ICC/IF, WBmore details -
种属反应性
与反应: Rat, Chicken, Human, Xenopus laevis, Chinese hamster
不与反应: Fish -
免疫原
Full length native protein (purified) corresponding to Chicken Talin 1 and 2.
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阳性对照
- WB: CHO cells ICC/IF: Chicken UMNSAH/DF1 cells
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常规说明
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR228677. Lot numbers higher than GR228677 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Purified from Tissue culture supernatant. -
克隆
单克隆 -
克隆编号
8D4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11188于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
See Abreview. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF | (1) |
Use at an assay dependent concentration.
See Abreview. |
WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa.
|
说明 |
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IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. See Abreview. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa. |
靶标
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相关性
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Talin 2 is related to talin 1. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. -
细胞定位
Cell projection, ruffle membrane (talin 1), Focal adhesion plaques and synapses (talin 2) -
数据库链接
- Entrez Gene: 395194 Chicken
- Entrez Gene: 7094 Human
- Entrez Gene: 83660 Human
- Entrez Gene: 313494 Rat
- Omim: 186745 Human
- Omim: 607349 Human
- SwissProt: P54939 Chicken
- SwissProt: Q8AWI0 Chicken
see all -
别名
- talin 1 antibody
- talin 2 antibody
- talin antibody
see all
图片
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Immunocytochemistry analysis of Chicken UMNSAH/DF1 cells labeling Talin 1 and 2 with ab11188 at 5 μg/mL. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG Atto-488 conjugate.
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Lane 1 : Anti-Talin 1 and 2 antibody [8D4] (ab11188) at 2 µg/ml
Lane 2 : Anti-Talin 1 and 2 antibody [8D4] (ab11188) at 1 mg/ml
All lanes : Whole extract of CHO cells
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 270 kDachemiluminescent substrate.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab11188 被引用在 16 文献中.
- Kao SH et al. Surface Viscosity-Dependent Neurite Initiation in Cortical Neurons. Adv Biol (Weinh) 6:e2101325 (2022). PubMed: 35362269
- Marhuenda E et al. Glioma stem cells invasive phenotype at optimal stiffness is driven by MGAT5 dependent mechanosensing. J Exp Clin Cancer Res 40:139 (2021). PubMed: 33894774
- Liu Q et al. Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABAA R-Dependent TRIM21 Modulation of Src Expression. Adv Sci (Weinh) 8:e2102079 (2021). PubMed: 34263559
- Son K et al. The eosinophil actin cytoskeleton undergoes rapid rearrangement in response to fluid shear stress. J Leukoc Biol N/A:N/A (2020). PubMed: 32349177
- Saleh A et al. A novel 3D nanofibre scaffold conserves the plasticity of glioblastoma stem cell invasion by regulating galectin-3 and integrin-ß1 expression. Sci Rep 9:14612 (2019). PubMed: 31601895