概述

  • 产品名称

    Anti-TAGLN/Transgelin抗体
    参阅全部 TAGLN/Transgelin 一抗
  • 描述

    山羊多克隆抗体to TAGLN/Transgelin
  • 宿主

    Goat
  • 经测试应用

    适用于: WB, Flow Cyt, IHC-Fr, IHC-P, IHC-FoFr, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Rabbit, Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human TAGLN/Transgelin aa 189-200 (C terminal).
    Sequence:

    C-MTGYGRPRQIIS


    (Peptide available as ab16067, ab23184)

  • 阳性对照

    • WB: Human muscle, heart, and placenta lysates. ICC/IF: REF52 and HeLa cells IHC-P: Human Uterus tissue, murine kidney tissue
  • 常规说明


    In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22-kD protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus. The protein is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

     This product was previously labelled as TAGLN

     

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明

    In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22-kD protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus. The protein is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

Primary incubation for 1 hour.

Preliminary testing was unsuccessful on Rat.

Flow Cyt Use at an assay dependent concentration. PubMed: 17099777

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 28955046
IHC-P 1/50. PubMed: 16080196
IHC-FoFr Use at an assay dependent concentration. PubMed: 26183159
ICC/IF Use a concentration of 5 µg/ml. PubMed: 22257561

靶标

  • 相关性

    Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
  • 细胞定位

    Cytoplasmic
  • 数据库链接

  • 别名

    • 22 kDa actin-binding protein antibody
    • Protein WS3-10 antibody
    • SM22 antibody
    • SM22-alpha antibody
    • Smooth muscle protein 22-alpha antibody
    • Transgelin antibody
    • WS3-10 antibody
    see all

图片

  • ab10135 (5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Anti-TAGLN/Transgelin antibody (ab10135) at 0.03 µg + Human Heart lysate at 1/35 dilution

    Predicted band size: 23 kDa

  • Immunofluorescence analysis of REF52 (rat embryo fibroblast) cells, staining SM22 alpha (green) with ab10135.

    Cells were fixed with paraformaldehyde for 10 minutes and permeabilized with 0.05% Triton X-100, before incubating with primary antibody. A fluorescent-conjugated anti-goat IgG was used as the secondary antibody.
  • Immunohistochemical analysis of PFA-fixed, paraffin embedded murine kidney tissue, staining SM22 alpha with ab10135 at 1/100 dilution.
  • Anti-TAGLN/Transgelin antibody (ab10135) at 0.5 µg/ml + Human muscle at 20 µg

    Secondary
    Donkey Anti-Goat IgG H&L (HRP) (ab6885) at 1/5000 dilution

    Predicted band size: 23 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa, 37 kDa, 42 kDa, 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes


    The band at 50kDa is recognised as contamination.
  • ICC/IF image of ab10135 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab10135 staining (0.2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab10135 staining (0.2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

  • Anti-TAGLN/Transgelin antibody (ab10135) at 0.02 µg/ml + Human Placenta lysate at 35 µg

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

文献

This product has been referenced in:

  • Wu W  et al. Vascular smooth muscle-MAPK14 is required for neointimal hyperplasia by suppressing VSMC differentiation and inducing proliferation and inflammation. Redox Biol 22:101137 (2019). Read more (PubMed: 30771750) »
  • Christensen ST  et al. MEK1/2 inhibitor U0126, but not nimodipine, reduces upregulation of cerebrovascular contractile receptors after subarachnoid haemorrhage in rats. PLoS One 14:e0215398 (2019). Read more (PubMed: 30978262) »
See all 84 Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews

Application
Western blot
Sample
Dog Tissue lysate - whole (heart valve)
Specification
heart valve
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: n/a

Abcam user community

Verified customer

提交于 Aug 15 2005

Application
Western blot
Sample
Rat Tissue lysate - whole (heart)
Specification
heart
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: N/A

Abcam user community

Verified customer

提交于 Aug 12 2005

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