为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-TAG72抗体[B72.3] (ab691)

  • Datasheet
  • SDS
Submit a review Q&A (5)References (15)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAG72  antibody [B72.3] (ab691)

    Key features and details

    • Mouse monoclonal [B72.3] to TAG72
    • Suitable for: IHC-P, IHC-Fr
    • Reacts with: Rat, Hamster, Human
    • Isotype: IgG1

    你可能会感兴趣

    一抗
    Product image
    Anti-Sialyl Tn antibody [STn 219] (ab115957)
    生物化学
    Product image
    CHIR99021, GSK3 inhibitor (ab120890)
    二抗
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    查看更多相关产品

    概述

    • 产品名称

      Anti-TAG72抗体[B72.3]
    • 描述

      小鼠单克隆抗体[B72.3] to TAG72
    • 宿主

      Mouse
    • 特异性

      This antibody recognises an oncofetal antigen of >1,000kDa, identified as a Tumor-Associated Glycoprotein (TAG-72) with the properties of a mucin. Weak or no reactivity has been observed with most cell types of normal adult tissue excepting the secretory endometrium.
    • 经测试应用

      适用于: IHC-P, IHC-Frmore details
    • 种属反应性

      与反应: Rat, Hamster, Human
    • 免疫原

      Tissue, cells or virus corresponding to TAG72. Membrane-enriched fraction of a human breast carcinoma liver metastasis

    • 阳性对照

      • Lung carcinoma or breast cancer.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituent: 99% Water

      Proprietary preservative that is not sodium azide or thimerosal, protein carrier.
    • 无载体

      是
    • Concentration information loading...
    • 克隆

      单克隆
    • 克隆编号

      B72.3
    • 骨髓瘤

      P3-NS1/1-Ag4-1
    • 同种型

      IgG1
    • 轻链类型

      kappa
    • 研究领域

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • Tumor Glycoproteins
      • Cancer
      • Tumor biomarkers
      • Tumor antigens

    相关产品

    • Antibody Diluent

      • Universal antibody diluent (ab79995)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Unmasking reagent

      • Trypsin Antigen Retrieval Solution (ab970)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab691于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Incubation for 30-60 minutes at room temperature is recommended.
    IHC-Fr
    Use at an assay dependent concentration.
    说明
    IHC-P
    1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Incubation for 30-60 minutes at room temperature is recommended.
    IHC-Fr
    Use at an assay dependent concentration.

    靶标

    • 相关性

      The majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary, and non-small cell lung cancer display some cell populations that are positive for TAG72. About 60% of carcinoma patients express TAG72 in their sera. TAG72 is reportedly useful in distinguishing pulmonary adenocarcinomas from pleural mesotheliomas.
    • 细胞定位

      Cytoplasmic
    • 别名

      • CA 72.4 antibody
      • CA72.4 antibody
      • TAG 72 antibody
      • Tumor associated glycoprotein 72 antibody

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAG72  antibody [B72.3] (ab691)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAG72 antibody [B72.3] (ab691)

      Immunohistochemical analysis of human colon carcinoma tissue labelled for TAG72 using ab691. Positive membranous staining observed.  

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (15)

    发表研究结果有使用 ab691?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab691 被引用在 15 文献中.

    • Cotton S  et al. Target Score-A Proteomics Data Selection Tool Applied to Esophageal Cancer Identifies GLUT1-Sialyl Tn Glycoforms as Biomarkers of Cancer Aggressiveness. Int J Mol Sci 22:N/A (2021). PubMed: 33562270
    • Persson N  et al. Epitope mapping of a new anti-Tn antibody detecting gastric cancer cells. Glycobiology 27:635-645 (2017). PubMed: 28419225
    • Tamura F  et al. RNAi-mediated gene silencing of ST6GalNAc I suppresses the metastatic potential in gastric cancer cells. Gastric Cancer 19:85-97 (2016). PubMed: 25532910
    • Xu F  et al. Expression profile of mucin-associated sialyl-Tn antigen in Chinese patients with different colorectal lesions (adenomas, carcinomas). Int J Clin Exp Pathol 8:11549-54 (2015). PubMed: 26617889
    • van Niekerk CC  et al. Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies. Cancer Detect Prev 21:247-57 (1997). PubMed: 9167042
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Question

    What is the concentration? What is in the buffer?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 09 2011

    Answer

    Thank you for your phone call this week and for your patience while I looked into your questions. I have heard back from the production lab of ab691, and the IgG concentrations vary among the lots that we have in stock: 60ug/mL for lot # 958786 33.3ug/mL for lots # 958785, 958787, 975535, and 975468   The protein carrier in the concentrated antibody diluent is BSA and the preservative is sodium azide.  I hope this information is helpful, but please let me know if you have any further questions.

    Read More

    Abcam Scientific Support

    回复于 Jun 09 2011

    Question

    Pls tell us the concentration of ab691 and ab16838. how many antibody contained in one package of 500 ul? Best regards > Dear Miss Liu, > > In response to your enquiry (ref: 808960): > Why not make customized products for us?Pls let me know the reason. > I am also wondering why not take this chance to make money. > > If you are wondering about the after-sales complaint,now I can confirm you we will never complanit about these customized products because it is us to ask you to do this. > > If you are wondering about the payment,we can prepay the charges immediately to yr account. > > Pls try your best to produce them for us. > > > We have an answer: > I am sorry but we are unable to process custom requests. We are only able to sell the products that we have and we are unable to send a sample. Please consider purchasing the antibodies that we have. We guarantee that they will work as stated on the datasheet. Thank you for your understanding. > > > Best regards > Bill > > > Bill Campbell, PhD > > Technical Support Manager > Abcam, Inc > https://www.abcam.com > > > > > > (CRM808960) > > >

    Read More

    Abcam community

    Verified customer

    Asked on Apr 21 2006

    Answer

    Thank you for your reply. Both of these antibodies are tissue culture supernatant meaning that it is not possible to determine antibody concentration, since tissue culture supernatant contains proteins besides the antibody of interest. Supernatant is the fluid resulting from centrifugation of hybridomas in tissue culture that are secreting specific monoclonal antibodies. Supernatants therefore typically contain culture medium, 5-10% fetal calf serum, and other proteins. Antibody concentration is only relevant for purified antibodies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Apr 24 2006

    Question

    Dear Sir or Madam: I want to 200ug of ab691 TAG72 antibody [B72.3] But its datasheet show 500ul, no concentration, Could you check and reply me?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 11 2006

    Answer

    Thank you for your enquiry. There's no concentration information available for this antibody as it is provided in an unpurified form (tissue culture supernatant) and we cannot determine the concentration for unpurified products. To use the antibody in IHC-P, it is recommended to dilute the antibody 1/50-1/100. Please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Apr 11 2006

    Question

    What is the concentration?

    Read More

    Abcam community

    Verified customer

    Asked on May 17 2005

    Answer

    Thank you for your enquiry. The concentration of the lots that we currently have in stock is 66 ug/ml. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 May 23 2005

    Question

    I have purchased two different lots of this antibody. Lots #48242 and 74306. This lots are behaving differently in my ELISA protocol. Do you have any information about these two lots that would account for this occurence?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 22 2004

    Answer

    Thank you for your enquiry. We are very sorry to hear that you are having some difficulty with this product. We would like to confirm that the two batches you are having are different, however we have not received any technical enquiry which would indicate that there is some batch-related problem. We would like to emphasize that this product has not been characterised for ELISA application only for IHC staining. We hope this information will be useful.

    Read More

    Abcam Scientific Support

    回复于 Oct 25 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2022 艾博抗(上海)贸易有限公司版权所有