Anti-TAF8/TBN抗体(ab204894)
Key features and details
- Rabbit polyclonal to TAF8/TBN
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-TAF8/TBN抗体 -
描述
兔多克隆抗体to TAF8/TBN -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis -
免疫原
Recombinant fragment corresponding to Human TAF8/TBN aa 50-200.
Database link: Q7Z7C8 -
阳性对照
- HEK lysate; Human placenta tissue; U-251 MG cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab204894于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 34 kDa.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 34 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo. -
序列相似性
Belongs to the TAF8 family.
Contains 1 histone-fold domain. -
结构域
Ectopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition. -
细胞定位
Nucleus. Cytoplasm. Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell. - Information by UniProt
-
数据库链接
- Entrez Gene: 419929 Chicken
- Entrez Gene: 539938 Cow
- Entrez Gene: 129685 Human
- Entrez Gene: 63856 Mouse
- Entrez Gene: 380201 Xenopus laevis
- Entrez Gene: 549471 Xenopus tropicalis
- Omim: 609514 Human
- SwissProt: Q5ZMS1 Chicken
see all -
别名
- FLJ32821 antibody
- hTAFII43 antibody
- Protein taube nuss antibody
see all
图片
-
All lanes : Anti-TAF8/TBN antibody (ab204894) at 1/100 dilution
Lane 1 : Negative Control
Lane 2 : HEK293 lysate
Predicted band size: 34 kDa -
Immunofluorescent analysis of U-251 MG cells (PFA-fixed/Triton X-100 permeabilized) labeling TAF8/TBN with ab204894 at 4 µg/ml (green).
-
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TAF8/TBN with ab204894 at 1/50 dilution.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab204894 被引用在 1 文献中.
- Fant CB et al. TFIID Enables RNA Polymerase II Promoter-Proximal Pausing. Mol Cell 78:785-793.e8 (2020). PubMed: 32229306