Key features and details
- Mouse monoclonal [STX01 (HPC-1)] to Syntaxin
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
参阅全部 Syntaxin 一抗
描述小鼠单克隆抗体[STX01 (HPC-1)] to Syntaxin
特异性In hippocampus, the antibody recognizes the stratum radiatum, the layer formed by the fibers of denate granule cells, as they synapse onto hippocampal pyramidal cells. In rat retina, it stains the membrane of the amacrine cell bodies and inner plexiform layer. It recognizes some neurons early in development and can be detected on migrating amacrine cells in embryonic retina.
经测试应用适用于: WB, ICC/IFmore details
种属反应性与反应: Rat, Human
Recombinant fragment corresponding to Rat Syntaxin aa 3-225.
- IMR-5 cells Cerebral cortex tissue sections. SH-SY5Y cell lysate
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
纯度Protein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab3265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Detects a band of approximately 31 kDa (predicted molecular weight: 35 kDa).|
功能Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
序列相似性Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
翻译后修饰Phosphorylated by CK2.
- Information by UniProt
- EPIM antibody
- Epimorphin antibody
- EPM antibody
All lanes : Anti-Syntaxin antibody [STX01 (HPC-1)] (ab3265) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 3 : Spinal Cord (Rat) Tissue Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
ICC/IF image of ab3265 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab3265 被引用在 9 文献中.
- Joussain C et al. Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons. Toxins (Basel) 11:N/A (2019). PubMed: 30791373
- Toft-Bertelsen TL et al. Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters. Mol Biol Cell 27:3329-3341 (2016). WB ; Mouse . PubMed: 27605709
- Shen C et al. The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis. Nat Commun 6:8852 (2015). PubMed: 26572858
- Mead B et al. Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts. PLoS One 9:e110612 (2014). IHC . PubMed: 25343338
- Yun HJ et al. LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. Exp Mol Med 45:e36 (2013). PubMed: 23949442
- Kunzevitzky NJ et al. Amacrine cell subtypes differ in their intrinsic neurite growth capacity. Invest Ophthalmol Vis Sci 54:7603-13 (2013). IHC ; Rat . PubMed: 24130183
- Kunzevitzky NJ et al. Foxn4 is required for retinal ganglion cell distal axon patterning. Mol Cell Neurosci 46:731-41 (2011). IHC ; Mouse . PubMed: 21334440
- Benagiano V et al. VAMP-2, SNAP-25A/B and syntaxin-1 in glutamatergic and GABAergic synapses of the rat cerebellar cortex. BMC Neurosci 12:118 (2011). IHC-P ; Rat . PubMed: 22094010
- Kunzevitzky NJ et al. Amacrine cell gene expression and survival signaling: differences from neighboring retinal ganglion cells. Invest Ophthalmol Vis Sci 51:3800-12 (2010). ICC/IF ; Rat . PubMed: 20445109