概述

  • 产品名称
    Anti-Syntaxin 4抗体[EPR15473]
    参阅全部 Syntaxin 4 一抗
  • 描述
    兔单克隆抗体[EPR15473] to Syntaxin 4
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Syntaxin 4 aa 100-200. The exact sequence is proprietary.
    Database link: Q12846

  • 阳性对照
    • WB: HEK-293, K562, HeLa and A431 cell lysates. Wild-type HAP1 cell lysate.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab184545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

靶标

  • 功能
    Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.
  • 组织特异性
    Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.
  • 序列相似性
    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NY REN 31 antigen antibody
    • p35 2 antibody
    • PLACENTAL antibody
    • Renal carcinoma antigen NY REN 31 antibody
    • Renal carcinoma antigen NY-REN-31 antibody
    • Stx4 antibody
    • STX4_HUMAN antibody
    • STX4A antibody
    • Syntaxin 4 antibody
    • syntaxin 4A (placental) antibody
    • Syntaxin 4A antibody
    • Syntaxin-4 antibody
    see all

图片

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Syntaxin 4 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab184545 observed at 1X kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab184545 was shown to recognize Syntaxin 4 when Syntaxin 4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Syntaxin 4 knockout samples were subjected to SDS-PAGE. ab184545 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/2000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/10000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

实验方案

文献

This product has been referenced in:
  • Machitani M  et al. MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2. J Virol 91:N/A (2017). WB ; Human . Read more (PubMed: 28356525) »
See 1 Publication for this product

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