重组Anti-Syntaxin 1a抗体[EPR11073(B)] (ab170889)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11073(B)] to Syntaxin 1a
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Syntaxin 1a抗体[EPR11073(B)]
参阅全部 Syntaxin 1a 一抗 -
描述
兔单克隆抗体[EPR11073(B)] to Syntaxin 1a -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human cerebellum and fetal brain lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11073(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170889于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 33 kDa. |
IP
1/10 - 1/100. |
靶标
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功能
Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. -
组织特异性
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. -
疾病相关
Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
序列相似性
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
细胞定位
Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 6804 Human
- Entrez Gene: 20907 Mouse
- Entrez Gene: 116470 Rat
- Omim: 186590 Human
- SwissProt: Q16623 Human
- SwissProt: O35526 Mouse
- SwissProt: P32851 Rat
- Unigene: 647024 Human
see all -
别名
- HPC 1 antibody
- Neuron specific antigen HPC1 antibody
- Neuron-specific antigen HPC-1 antibody
see all
图片
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Western blot analysis on immunoprecipitation pellet from human fetal brain lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170889 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Lane 1 Primary: ab170889, 1/1000 dilution
Sample: Immunoprecipitated Human fetal brain lysate
Secondary: HRP-conjugated anti-rabbit IgGLane 2 Primary: ab170889, 1/1000 dilution
Sample: 1X PBS (negative control)
Secondary: HRP-conjugated anti-rabbit IgGPredicted band 33 kDa
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All lanes : Anti-Syntaxin 1a antibody [EPR11073(B)] (ab170889) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab170889 被引用在 2 文献中.
- Shumate KM et al. RNA editing-mediated regulation of calcium-dependent activator protein for secretion (CAPS1) localization and its impact on synaptic transmission. J Neurochem 158:182-196 (2021). PubMed: 33894004
- Gao W et al. IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury. Front Neurol 8:281 (2017). PubMed: 28713327