Key features and details
- Rabbit polyclonal to Synphilin 1
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 Synphilin 1 一抗
描述兔多克隆抗体to Synphilin 1
特异性Immunohistochemical analysis of human brain indicates a high level of specificity for this antiserum. Cross reactivity: This antibody is known to react with rat and human. Cross reactivity with other species has not been tested yet.
经测试应用适用于: IHC-Pmore details
种属反应性与反应: Mouse, Rat, Human
This antibody stains Lewy bodies in Dorsal raphe nucleus of Parkinson’s diseased human brain.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Our Abpromise guarantee covers the use of ab6179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
功能Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.
组织特异性Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.
序列相似性Contains 6 ANK repeats.
翻译后修饰Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation.
细胞定位Cytoplasm. Detected in cytoplasmic inclusion bodies, together with SNCA.
- Information by UniProt
- Alpha synuclein interacting protein antibody
- Alpha-synuclein-interacting protein antibody
- MGC39814 antibody
IHC image of ab6179 staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab6179 被引用在 3 文献中.
- Liang X et al. Panaxadiol inhibits synaptic dysfunction in Alzheimer's disease and targets the Fyn protein in APP/PS1 mice and APP-SH-SY5Y cells. Life Sci 221:35-46 (2019). PubMed: 30735733
- Kühn K et al. Parkin expression in the developing mouse. Brain Res Dev Brain Res 149:131-42 (2004). PubMed: 15063093
- Specht CG & Schoepfer R Deletion of the alpha-synuclein locus in a subpopulation of C57BL/6J inbred mice. BMC Neurosci 2:11 (2001). PubMed: 11591219