Anti-Synaptophysin抗体(ab14692)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Synaptophysin抗体
参阅全部 Synaptophysin 一抗 -
描述
兔多克隆抗体to Synaptophysin -
宿主
Rabbit -
特异性
The antibody does not cross-react other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Synaptophysin aa 41-62.
Sequence:FATCGSYSGELQLSVDCANKTE
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阳性对照
- IHC: Rat and mouse brain. WB: Mouse forebrain extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
The buffer includes stabilizing proteins and glycerol. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14692于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
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IHC-P | (2) |
1/100.
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说明 |
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WB
1/200 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/100. |
靶标
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功能
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
组织特异性
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
疾病相关
Mental retardation, X-linked, SYP-related -
序列相似性
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
结构域
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- SwissProt: Q62277 Mouse
- SwissProt: P07825 Rat
- Unigene: 223674 Mouse
- Unigene: 11067 Rat
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别名
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
图片
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Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded mouse brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
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Synaptophysin antibody (ab14692) western blot on mouse forebrain extract (10µg protein/lane). The blot was exposed for 10 minutes to autoradiographic film. Ladder markers from 7-194 kDa.
Lane 1 = 1/500 ab14692
Lane 2 = 1/200 ab14692 -
Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded rat brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (102)
ab14692 被引用在 102 文献中.
- Mason MRJ et al. The Jun-dependent axon regeneration gene program: Jun promotes regeneration over plasticity. Hum Mol Genet 31:1242-1262 (2022). PubMed: 34718572
- Xu Y et al. Ketogenic diet ameliorates cognitive impairment and neuroinflammation in a mouse model of Alzheimer's disease. CNS Neurosci Ther 28:580-592 (2022). PubMed: 34889516
- Scaramuzzino C et al. Calcineurin and huntingtin form a calcium-sensing machinery that directs neurotrophic signals to the nucleus. Sci Adv 8:eabj8812 (2022). PubMed: 34985962
- Dentoni G et al. Mitochondria-Endoplasmic Reticulum Interplay Regulates Exo-Cytosis in Human Neuroblastoma Cells. Cells 11:N/A (2022). PubMed: 35159324
- Xie Z et al. Microglial cathepsin E plays a role in neuroinflammation and amyloid β production in Alzheimer's disease. Aging Cell 21:e13565 (2022). PubMed: 35181976