Anti-Synapsin I抗体- Synaptic Marker (ab8)

Reviews (3)Q&A (8)References (38)

Key features and details

  • Rabbit polyclonal to Synapsin I - Synaptic Marker
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Guinea pig, Cow, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Synapsin I抗体- Synaptic Marker
    参阅全部 Synapsin I 一抗

  • 描述

    兔多克隆抗体to Synapsin I - Synaptic Marker

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Guinea pig, Cow, Human

  • 免疫原

    Full length native protein (purified) corresponding to Cow Synapsin I.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab8 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 80 kDa.

靶标

  • 功能

    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

  • 疾病相关

    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.

  • 序列相似性

    Belongs to the synapsin family.

  • 翻译后修饰

    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 细胞定位

    Cell junction > synapse. Golgi apparatus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

图片

文献 (38)

发表研究结果有使用 ab8？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab8 被引用在 38 文献中.

  • De Paepe B  et al. Myo-Inositol Transporter SLC5A3 Associates with Degenerative Changes and Inflammation in Sporadic Inclusion Body Myositis. Biomolecules 10:N/A (2020). PubMed: 32235474
  • Liu X  et al. miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model. Int J Mol Med 44:1729-1740 (2019). PubMed: 31545395
  • Siemsen BM  et al. Effects of Methamphetamine Self-Administration and Extinction on Astrocyte Structure and Function in the Nucleus Accumbens Core. Neuroscience 406:528-541 (2019). PubMed: 30926546
  • Kullmann FA  et al. Serotonergic paraneurones in the female mouse urethral epithelium and their potential role in peripheral sensory information processing. Acta Physiol (Oxf) 222:N/A (2018). PubMed: 28719042
  • Hansen SN  et al. Long term Westernized diet leads to region-specific changes in brain signaling mechanisms. Neurosci Lett 676:85-91 (2018). WB ; Guinea pig . PubMed: 29655945
View all Publications for this product

客户评价及客户问答

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1-10 of 11 Abreviews or Q&A

Immunocytochemistry abreview for Anti-Synapsin I antibody - Synaptic Marker

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (neurons derived from iPSCs)
Specification
neurons derived from iPSCs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Apr 13 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synapsin I antibody - Synaptic Marker

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (fixed mouse brain)
Specification
fixed mouse brain
Permeabilization
Yes - 0.5% TrintonX-100
Fixative
Paraformaldehyde
Read More

Dr. Hyun Jin Kim

Verified customer

提交于 Aug 20 2013

Question

How many days do you recommend culturing primary neurons to get a good Synapsin I signal? Can you please send the protocol used to validate this antibody in immunofluorescence?

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Answer

Thank you for your patience. The IF validation for this antibody was done by a collaborator, so unfortunately we do not have a specific protocol to provide. The neurons were cultured for 14 days. I hope this helps, please let me know if you need any additional information or assistance.

Read More

Question

No pulldown in IP using mouse and human samples.

Read More
Answer

Thank you for your call yesterday and for letting us know about the trouble with ab8 in IP.
I've attached the specific IP protocol for ab8, which was optimized with Sacc cells. I am hopeful that this protocol will be a good referece, but if you would like to send some details about your experiment I will also see if there are any other suggestions to make.
Please keep me updated about the results using the replacement vial, and I let me know if you have any questions or if there is anything else that I can do for you.

Read More

Question

Recently, I've been doing some experiments with some colleagues on synapsin 1.
I have purchased 2 antibodies from abcam: one N-ter (ab18814) for WB and one (ab8) for IF.

The ab18814 is working great! Do you think you could tell me the amino acids recognized by this antibody? my colleagues have performed MALDI technique and they need to make sure that the peptide captured by proteomic is the same than the one seen in WB.

For IF, unfortunately, the ab8 gave poor results on mouse fixed brain sections, with small numbers of neurons stained (ab18814 didn't work on IF neither).
Do you have any recommendations for another ab to test? Maybe ab64581?

Read More
Answer

Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement. Ab64581 has been tested in IHC and reacts with mouse, and has very positive reviews. I've included a link to the datasheet below so you can take a look and make sure this is the one you want. I'm happy to send you a vial free of charge.

For ab18814, this product is produced by a third party so we do not have the exact immunogen sequence. We do know that the immunogen peptide is taken from around amino acid 12. In my experience, immunogen peptides are normallysomewhere around 15-20 amino acids long, but obviously this could be different for this antibody. I'm going to see how much information I can get from the originator of the antibody, but I am not sure whether they will release the exact sequence to us.

Please let me know if you'd like a vial of ab64581 and I will set the order up for you.

I look forward to your reply. Have a great day!

Read More

Question

Ok, please send me a vial of ab18258. Have a good weekend.  

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab18258 with the order number 996056. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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Question

Thank you for your answer. I prefer to get a replacement antibody, but I am not which one is more specific for synapse staining in your company. Could you give some suggestion? Thank you very much!  

Read More
Answer

Thanks for your reply. One example of a popular target for synapse staining is PSD95. A good antibody to detect this protein in a variety of applications and species is ab18258. I would be happy to send you a vial of ab18258 if you are interested. Please check the online datasheet for this product at the link below to make sure it is tested in the applications and species you are working with: https://www.abcam.com/PSD95-antibody-Synaptic-Marker-ab18258.html I hope this is helpful. I look forward to hearing from you.

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Synapsin I antibody - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cultured Cells (Hippocampal neurons from P0 /P1)
Specification
Hippocampal neurons from P0 /P1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Read More

Dr. Senthil Thyagarajan

Verified customer

提交于 Feb 11 2010

Question

I haved got an antibody(rabbit polyclonal to synapsin I--ab8) from you company.The application notes in datasheet tells me that the recommended dilutions for WB is 1:200;but it is used at a concentration of 0.1ug/ml(1:2000)for IHC-F.Normally,we use the higher dilution for WB than for IHC(maybe 1:3000 or higher). So i am not sure if the recommended dilution 1:200 for WB in the datasheet is correct.Please tell me the recommended dilution for WB. Could you please confirm the recommended dilutions for WB?

Read More
Answer

Thank you for your enquiry. It seems that we had an error in our datasheet. The recommended dilution for WB should read as "WB: 1/1000". Please rest assure that the datasheet has already been updated to show the changes. Thank you for informing us about this and I sincerely apologize for the inconvenience. Should you require any further assistance, please do not hesitate to contact us.

Read More

Question

I recently ordered a vial of pAb to Synapsin1(Novus), NB300-104 (lot 1650 304kn), 10 ug reconstitute in 50 ul. We tried on frozen sections as well as paraffin sections. Nothing worked! We even use pretty high concentration, 1/20 dilution. Will you kindly help me find a reliable technique to do the work. References will also be most welcome!

Read More
Answer

I'm sorry to hear that you are having trouble getting this antibody to work in your research. The antibody is suitable for use in IHC on both frozen and fixed sections and Novus recommend a dilution of 1:1000. There is a reference on the datasheet that features the use of this antibody, but I'm not sure whether or not that was in IHC: Danglot L et al. Association of gephyrin with synaptic and extrasynaptic GABAA receptors varies during development in cultured hippocampal neurons. Mol Cell Neurosci 23:264-78 (2003). PubMed: 12812758. In order to help you further I will need more details of your protocol and the nature of the material on which you are working. The technical help section of our website will be useful to you as it contains many helpful suggestions as to how you could optimise your protocol. Go to the Abcam homepage www.abcam.com and type in ab8 into the search box. Once on the datasheet, click through to the enquiries tab and onto the link for technical support. You can specify that you are using the antibody in IHC. If this does not help, please complete the form to tell us more about how you are using the antibody.

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1-10 of 11 Abreviews or Q&A

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