概述

  • 产品名称
    Anti-Synapsin I抗体- Synaptic Marker
    参阅全部 Synapsin I 一抗
  • 描述
    兔多克隆抗体to Synapsin I - Synaptic Marker
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC, ICC/IF, WB, IP, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow Synapsin I.

  • 阳性对照
    • WB: Rat brain tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab8 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/500.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 80 kDa.
IP Use at an assay dependent concentration. 1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.
IHC-FoFr Use a concentration of 0.1 µg/ml.
IHC-Fr 1/1000.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.

靶标

  • 功能
    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
  • 疾病相关
    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
  • 序列相似性
    Belongs to the synapsin family.
  • 翻译后修饰
    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cell junction > synapse. Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

图片

  • Anti-Synapsin I antibody - Synaptic Marker (ab8) + Rat brain tissue lysate at 10 µg

    Predicted band size: 80 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?

  • Mouse hippocampal primary neurons were fixed, permeabilised and
    labeled with synapsin I antibody ab8 for 1 h at a dilution of 1:500, followed by FITC-conjugated anti rabbit IgG (left panel, green). Co-staining of the same specimen with another antibody against synapsin I (Cy3-conjugated secondary antibody; middle panel, red) confirmed the specificity of both antibodies for the antigen (right panel, yellow colocalisation).

    These images were kindly supplied as part of the review submitted by Christian Specht.

  • ab8 staining Synapsin I in rat Hippocampal neurons from PO/P1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.1 % Triton. Samples were incubated with primary antibody, diluted 1/400, for 12 hours at 40C. An AMCA-conjugated donkey polyclonal to rabbit IgG was used at 1/500 dilution as secondary antibody.

    See Abreview

  • Human Cultured Cells (neurons derived from iPSCs) stained for Synapsin I (red) using ab8 (1/400 dilution) and beta III Tubulin (green) in ICC.

文献

This product has been referenced in:
  • Hansen SN  et al. Long term Westernized diet leads to region-specific changes in brain signaling mechanisms. Neurosci Lett 676:85-91 (2018). WB ; Guinea pig . Read more (PubMed: 29655945) »
  • Hansen SN  et al. Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. Nutrients 10:N/A (2018). Read more (PubMed: 29890692) »
See all 31 Publications for this product

客户评价及客户问答

1-10 of 11 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Human Cultured Cells (neurons derived from iPSCs)
Specification
neurons derived from iPSCs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Apr 13 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (fixed mouse brain)
Specification
fixed mouse brain
Permeabilization
Yes - 0.5% TrintonX-100
Fixative
Paraformaldehyde

Dr. Hyun Jin Kim

Verified customer

提交于 Aug 20 2013

Answer

Thank you for your patience. The IF validation for this antibody was done by a collaborator, so unfortunately we do not have a specific protocol to provide. The neurons were cultured for 14 days. I hope this helps, please let me know if you need any additional information or assistance.

Read More

Question
Answer

Thank you for your call yesterday and for letting us know about the trouble with ab8 in IP.
I've attached the specific IP protocol for ab8, which was optimized with Sacc cells. I am hopeful that this protocol will be a good referece, but if you would like to send some details about your experiment I will also see if there are any other suggestions to make.
Please keep me updated about the results using the replacement vial, and I let me know if you have any questions or if there is anything else that I can do for you.

Read More

Answer

Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement. Ab64581 has been tested in IHC and reacts with mouse, and has very positive reviews. I've included a link to the datasheet below so you can take a look and make sure this is the one you want. I'm happy to send you a vial free of charge.

For ab18814, this product is produced by a third party so we do not have the exact immunogen sequence. We do know that the immunogen peptide is taken from around amino acid 12. In my experience, immunogen peptides are normallysomewhere around 15-20 amino acids long, but obviously this could be different for this antibody. I'm going to see how much information I can get from the originator of the antibody, but I am not sure whether they will release the exact sequence to us.

Please let me know if you'd like a vial of ab64581 and I will set the order up for you.

I look forward to your reply. Have a great day!

Read More

Question
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab18258 with the order number 996056. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More

Answer

Thanks for your reply. One example of a popular target for synapse staining is PSD95. A good antibody to detect this protein in a variety of applications and species is ab18258. I would be happy to send you a vial of ab18258 if you are interested. Please check the online datasheet for this product at the link below to make sure it is tested in the applications and species you are working with: https://www.abcam.com/PSD95-antibody-Synaptic-Marker-ab18258.html I hope this is helpful. I look forward to hearing from you.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cultured Cells (Hippocampal neurons from P0 /P1)
Specification
Hippocampal neurons from P0 /P1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton

Dr. Senthil Thyagarajan

Verified customer

提交于 Feb 11 2010

Answer

Thank you for your enquiry. It seems that we had an error in our datasheet. The recommended dilution for WB should read as "WB: 1/1000". Please rest assure that the datasheet has already been updated to show the changes. Thank you for informing us about this and I sincerely apologize for the inconvenience. Should you require any further assistance, please do not hesitate to contact us.

Read More

Answer

I'm sorry to hear that you are having trouble getting this antibody to work in your research. The antibody is suitable for use in IHC on both frozen and fixed sections and Novus recommend a dilution of 1:1000. There is a reference on the datasheet that features the use of this antibody, but I'm not sure whether or not that was in IHC: Danglot L et al. Association of gephyrin with synaptic and extrasynaptic GABAA receptors varies during development in cultured hippocampal neurons. Mol Cell Neurosci 23:264-78 (2003). PubMed: 12812758. In order to help you further I will need more details of your protocol and the nature of the material on which you are working. The technical help section of our website will be useful to you as it contains many helpful suggestions as to how you could optimise your protocol. Go to the Abcam homepage www.abcam.com and type in ab8 into the search box. Once on the datasheet, click through to the enquiries tab and onto the link for technical support. You can specify that you are using the antibody in IHC. If this does not help, please complete the form to tell us more about how you are using the antibody.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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