Anti-Synapsin I抗体- Synaptic Marker (ab8)
概述
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产品名称
Anti-Synapsin I抗体- Synaptic Marker
参阅全部 Synapsin I 一抗 -
描述
兔多克隆抗体to Synapsin I - Synaptic Marker -
宿主
Rabbit -
经测试应用
适用于: ICC, ICC/IF, WB, IP, IHC-FoFr, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Guinea pig, Cow, Human -
免疫原
Full length native protein (purified) corresponding to Cow Synapsin I.
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阳性对照
- WB: Rat brain tissue lysate
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituents: 0.24% HEPES, 0.87% Sodium chloride, 0.0001% BSA, 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab8 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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ICC | 1/500. | |
ICC/IF | Use at an assay dependent concentration. | |
WB | 1/1000. Predicted molecular weight: 80 kDa. | |
IP | Use at an assay dependent concentration. 1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain. | |
IHC-FoFr | Use a concentration of 0.1 µg/ml. | |
IHC-Fr | 1/1000. | |
IHC-P | Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. |
靶标
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功能
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
疾病相关
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
序列相似性
Belongs to the synapsin family. -
翻译后修饰
Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cell junction > synapse. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 281510 Cow
- Entrez Gene: 6853 Human
- Entrez Gene: 20964 Mouse
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17599 Cow
- SwissProt: P17600 Human
- SwissProt: O88935 Mouse
see all -
别名
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
图片
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Anti-Synapsin I antibody - Synaptic Marker (ab8) + Rat brain tissue lysate at 10 µg
Predicted band size: 80 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody - Synaptic Marker (ab8)This image is courtesy of Christian Specht
Mouse hippocampal primary neurons were fixed, permeabilised and
labeled with synapsin I antibody ab8 for 1 h at a dilution of 1:500, followed by FITC-conjugated anti rabbit IgG (left panel, green). Co-staining of the same specimen with another antibody against synapsin I (Cy3-conjugated secondary antibody; middle panel, red) confirmed the specificity of both antibodies for the antigen (right panel, yellow colocalisation).These images were kindly supplied as part of the review submitted by Christian Specht.
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Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody - Synaptic Marker (ab8)This image is a courtesy of an Abreview submitted by Senthil Thyagarajan.
ab8 staining Synapsin I in rat Hippocampal neurons from PO/P1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.1 % Triton. Samples were incubated with primary antibody, diluted 1/400, for 12 hours at 40C. An AMCA-conjugated donkey polyclonal to rabbit IgG was used at 1/500 dilution as secondary antibody.
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Human Cultured Cells (neurons derived from iPSCs) stained for Synapsin I (red) using ab8 (1/400 dilution) and beta III Tubulin (green) in ICC.
实验方案
文献
This product has been referenced in:
- Xie X et al. ß-actin regulates a heterochromatin landscape essential for optimal induction of neuronal programs during direct reprograming. PLoS Genet 14:e1007846 (2018). Read more (PubMed: 30557298) »
- Sun H et al. A Purinergic P2 Receptor Family-Mediated Increase in Thrombospondin-1 Bolsters Synaptic Density and Epileptic Seizure Activity in the Amygdala-Kindling Rat Model. Front Cell Neurosci 12:302 (2018). Read more (PubMed: 30386206) »