Anti-Synapsin I抗体- Synaptic Marker (ab8)

Abreviews (3)Q&A (8)Specific References (29)

概述

  • 产品名称
    Anti-Synapsin I抗体- Synaptic Marker
    参阅全部 Synapsin I 一抗
  • 描述
    兔多克隆抗体to Synapsin I - Synaptic Marker
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC, ICC/IF, WB, IP, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow Synapsin I.

  • 阳性对照
    • WB: Rat brain tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab8 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/500.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 80 kDa.
IP Use at an assay dependent concentration. 1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.
IHC-FoFr Use a concentration of 0.1 µg/ml.
IHC-Fr 1/1000.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.

靶标

  • 功能
    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
  • 疾病相关
    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
  • 序列相似性
    Belongs to the synapsin family.
  • 翻译后修饰
    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cell junction > synapse. Golgi apparatus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

图片

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab8)
    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab8)
    Anti-Synapsin I antibody - Synaptic Marker (ab8) + Rat brain tissue lysate at 10 µg

    Predicted band size: 80 kDa
    Observed band size: 78 kDa (why is the actual band size different from the predicted?)

  • Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody - Synaptic Marker (ab8)
    Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody - Synaptic Marker (ab8)This image is courtesy of Christian Specht

    Mouse hippocampal primary neurons were fixed, permeabilised and
    labeled with synapsin I antibody ab8 for 1 h at a dilution of 1:500, followed by FITC-conjugated anti rabbit IgG (left panel, green). Co-staining of the same specimen with another antibody against synapsin I (Cy3-conjugated secondary antibody; middle panel, red) confirmed the specificity of both antibodies for the antigen (right panel, yellow colocalisation).

    These images were kindly supplied as part of the review submitted by Christian Specht.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody - Synaptic Marker (ab8)
    Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody - Synaptic Marker (ab8)This image is a courtesy of an Abreview submitted by Senthil Thyagarajan.

    ab8 staining Synapsin I in rat Hippocampal neurons from PO/P1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.1 % Triton. Samples were incubated with primary antibody, diluted 1/400, for 12 hours at 40C. An AMCA-conjugated donkey polyclonal to rabbit IgG was used at 1/500 dilution as secondary antibody.

    See Abreview

  • Immunocytochemistry - Anti-Synapsin I antibody - Synaptic Marker (ab8)
    Immunocytochemistry - Anti-Synapsin I antibody - Synaptic Marker (ab8)

    Human Cultured Cells (neurons derived from iPSCs) stained for Synapsin I (red) using ab8 (1/400 dilution) and beta III Tubulin (green) in ICC.

文献

This product has been referenced in:
  • Hansen SN  et al. Long term Westernized diet leads to region-specific changes in brain signaling mechanisms. Neurosci Lett 676:85-91 (2018). WB ; Guinea pig . Read more (PubMed: 29655945) »
  • Noller CM  et al. Structural Remodeling of Sympathetic Innervation in Atherosclerotic Blood Vessels: Role of Atherosclerotic Disease Progression and Chronic Social Stress. Psychosom Med 79:59-70 (2017). IF . Read more (PubMed: 27359178) »

See all 29 Publications for this product

发表研究结果有使用 ab8？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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11 reviews or Q&A

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Immunocytochemistry abreview for Anti-Synapsin I antibody - Synaptic Marker

 Good
Abreviews
Application
Immunocytochemistry
Sample
Human Cultured Cells (neurons derived from iPSCs)
Specification
neurons derived from iPSCs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Apr 13 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synapsin I antibody - Synaptic Marker

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (fixed mouse brain)
Specification
fixed mouse brain
Permeabilization
Yes - 0.5% TrintonX-100
Fixative
Paraformaldehyde
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Dr. Hyun Jin Kim

Verified customer

提交于 Aug 20 2013

How many days do you recommend culturing primary neurons to get a good Synapsin I signal? Can you please send the protocol used to validate this antibody in immunofluorescence?

Thank you for your patience. The IF validation for this antibody was done by a collaborator, so unfortunately we do not have a specific protocol to provide. The neurons were cultured for 14 days. I hope this helps, please let me know if you need any ad...

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No pulldown in IP using mouse and human samples.

Thank you for your call yesterday and for letting us know about the trouble with ab8 in IP.
I've attached the specific IP protocol for ab8, which was optimized with Sacc cells. I am hopeful that this protocol will be a good referece, but if you wo...

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Recently, I've been doing some experiments with some colleagues on synapsin 1.
I have purchased 2 antibodies from abcam: one N-ter (ab18814) for WB and one (ab8) for IF.

The ab18814 is working great! Do you think you could tell me the amin...

Read More

Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement....

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Ok, please send me a vial of ab18258. Have a good weekend.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab18258 with the order number 996056. To check the status of the orde...

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Thank you for your answer. I prefer to get a replacement antibody, but I am not which one is more specific for synapse staining in your company. Could you give some suggestion? Thank you very much!

Thanks for your reply. One example of a popular target for synapse staining is PSD95. A good antibody to detect this protein in a variety of applications and species is ab18258. I would be happy to send you a vial of ab18258 if you are interest...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Synapsin I antibody - Synaptic Marker

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cultured Cells (Hippocampal neurons from P0 /P1)
Specification
Hippocampal neurons from P0 /P1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Read More
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Dr. Senthil Thyagarajan

Verified customer

提交于 Feb 11 2010

I haved got an antibody(rabbit polyclonal to synapsin I--ab8) from you company.The application notes in datasheet tells me that the recommended dilutions for WB is 1:200;but it is used at a concentration of 0.1ug/ml(1:2000)for IHC-F.Normally,we use the...

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Thank you for your enquiry. It seems that we had an error in our datasheet. The recommended dilution for WB should read as "WB: 1/1000". Please rest assure that the datasheet has already been updated to show the changes. Thank you for informing...

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I recently ordered a vial of pAb to Synapsin1(Novus), NB300-104 (lot 1650 304kn), 10 ug reconstitute in 50 ul. We tried on frozen sections as well as paraffin sections. Nothing worked! We even use pretty high concentration, 1/20 dilution. Will yo...

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I'm sorry to hear that you are having trouble getting this antibody to work in your research. The antibody is suitable for use in IHC on both frozen and fixed sections and Novus recommend a dilution of 1:1000. There is a reference on the datasheet that...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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