Anti-Synapsin I抗体- Synaptic Marker (ab8)


  • 产品名称
    Anti-Synapsin I抗体- Synaptic Marker
    参阅全部 Synapsin I 一抗
  • 描述
    兔多克隆抗体to Synapsin I - Synaptic Marker
  • 宿主
  • 经测试应用
    适用于: ICC, ICC/IF, WB, IP, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow Synapsin I.

  • 阳性对照
    • WB: Rat brain tissue lysate



Our Abpromise guarantee covers the use of ab8 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/500.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 80 kDa.
IP Use at an assay dependent concentration. 1 ug will quantitatively immunoprecipitate all synapsin in 200 ug of rat brain.
IHC-FoFr Use a concentration of 0.1 µg/ml.
IHC-Fr 1/1000.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.


  • 功能
    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
  • 疾病相关
    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
  • 序列相似性
    Belongs to the synapsin family.
  • 翻译后修饰
    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Cell junction > synapse. Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all


  • Anti-Synapsin I antibody - Synaptic Marker (ab8) + Rat brain tissue lysate at 10 µg

    Predicted band size: 80 kDa
    Observed band size: 78 kDa (why is the actual band size different from the predicted?)

  • Mouse hippocampal primary neurons were fixed, permeabilised and
    labeled with synapsin I antibody ab8 for 1 h at a dilution of 1:500, followed by FITC-conjugated anti rabbit IgG (left panel, green). Co-staining of the same specimen with another antibody against synapsin I (Cy3-conjugated secondary antibody; middle panel, red) confirmed the specificity of both antibodies for the antigen (right panel, yellow colocalisation).

    These images were kindly supplied as part of the review submitted by Christian Specht.

  • ab8 staining Synapsin I in rat Hippocampal neurons from PO/P1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.1 % Triton. Samples were incubated with primary antibody, diluted 1/400, for 12 hours at 40C. An AMCA-conjugated donkey polyclonal to rabbit IgG was used at 1/500 dilution as secondary antibody.

    See Abreview

  • Human Cultured Cells (neurons derived from iPSCs) stained for Synapsin I (red) using ab8 (1/400 dilution) and beta III Tubulin (green) in ICC.


This product has been referenced in:
  • Hansen SN  et al. Long term Westernized diet leads to region-specific changes in brain signaling mechanisms. Neurosci Lett 676:85-91 (2018). WB ; Guinea pig . Read more (PubMed: 29655945) »
  • Noller CM  et al. Structural Remodeling of Sympathetic Innervation in Atherosclerotic Blood Vessels: Role of Atherosclerotic Disease Progression and Chronic Social Stress. Psychosom Med 79:59-70 (2017). IF . Read more (PubMed: 27359178) »

See all 29 Publications for this product


Human Cultured Cells (neurons derived from iPSCs)
neurons derived from iPSCs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Apr 13 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step
Mouse Tissue sections (fixed mouse brain)
fixed mouse brain
Yes - 0.5% TrintonX-100

Dr. Hyun Jin Kim

Verified customer

提交于 Aug 20 2013

Thank you for your patience. The IF validation for this antibody was done by a collaborator, so unfortunately we do not have a specific protocol to provide. The neurons were cultured for 14 days. I hope this helps, please let me know if you need any ad...

Read More

Thank you for your call yesterday and for letting us know about the trouble with ab8 in IP.
I've attached the specific IP protocol for ab8, which was optimized with Sacc cells. I am hopeful that this protocol will be a good referece, but if you wo...

Read More

Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement....

Read More

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab18258 with the order number 996056. To check the status of the orde...

Read More

Thanks for your reply. One example of a popular target for synapse staining is PSD95. A good antibody to detect this protein in a variety of applications and species is ab18258. I would be happy to send you a vial of ab18258 if you are interest...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Rat Cultured Cells (Hippocampal neurons from P0 /P1)
Hippocampal neurons from P0 /P1
Yes - 0.1% triton

Dr. Senthil Thyagarajan

Verified customer

提交于 Feb 11 2010

Thank you for your enquiry. It seems that we had an error in our datasheet. The recommended dilution for WB should read as "WB: 1/1000". Please rest assure that the datasheet has already been updated to show the changes. Thank you for informing...

Read More

I'm sorry to hear that you are having trouble getting this antibody to work in your research. The antibody is suitable for use in IHC on both frozen and fixed sections and Novus recommend a dilution of 1:1000. There is a reference on the datasheet that...

Read More

1-10 of 11 Abreviews or Q&A