为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Kits/ Lysates/ Other Lysates Whole Cell Lysates Human Other

SW480全细胞裂解物(ab3957)

  • Datasheet
  • SDS
Submit a review Q&A (1)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

概述

  • 产品名称

    SW480全细胞裂解物
  • 常规说明

    Cell line: SW480 (Human colorectal adenocarcinoma).
    Growth media: Leibovitz’s L-15 medium with 2 mM L-glutamine, and 10% FBS.


    SW480 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用

    适用于: WBmore details

性能

  • Mycoplasma free

    Yes
  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 裂解物说明

    SW480 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究领域

    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Human
    • Other
  • 背景

    Cells are epithelial like in morphology and used as an in vitro model for colorectal cancer to study tumor markers, CEA production, biochemistry of tumorigenicity, pharmacodynamics, hormone sensibility and as a positive control for the nuclear protein Beta catenin.

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Use at an assay dependent concentration. SW480 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane is recommended for mini gel.
说明
WB
Use at an assay dependent concentration. SW480 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane is recommended for mini gel.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (0)

发表研究结果有使用 ab3957?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3957 尚未被引用在任何文献中。

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

Question

Dear technical support team: This customer raises an enquiry about ab51236 (Anti-HOXA9 antibody [HOX5I043]), she wants to know is there any suggestion can help her to choice the suitable positive control of this antibody? Would you please help him to solve the problem? Thanks for your kindly help. Best regards

Read More

Abcam community

Verified customer

Asked on Jan 11 2012

Answer

Thank you for contacting us. We are happy to help customers find the most suitable positive controls for their research purposes; however we are not specialized in this particular field and do not want to recommend a control that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. A preliminary literature search showed that human colon adenocarcinoma should be a suitable positive control. A Human colon adenocarcinoma cell line SW480 was also found to be positive for HOXA9. We do offer a SW480 lysate for WB that could then be used as positive control: ab3957 (SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate) Click here (or use the following: https://www.abcam.com/SW480-whole-cell-lysate-ab3957.html). I also found that the following cells were found positive: 721_B cells and hep2G cells. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

Read More

Abcam Scientific Support

回复于 Jan 11 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2021 艾博抗(上海)贸易有限公司版权所有