参阅全部 SV40 T-antigen 一抗
描述小鼠单克隆抗体[PAb416] to SV40 T-antigen
经测试应用适用于: ICC, Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
种属反应性SV40 infected cells.
SV40 transformed mouse cells
表位Within amino acids 83-128 of the large T-antigen.
- SV80 or SV-T2 cells.
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab16879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 1 - 5 µg/ml.|
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.|
|IP||Use a concentration of 1 - 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody
All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
All lanes : HRP conjugated sheep-anti mouse IgG
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 82 kDa
Observed band size: ~80 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.
CV1 cells were either mock infected or infected with SV40 at moi 10. Cells were then harvested (trypsinized and centrifuged) and fixed 72 hours post infection in 100% Methanol at -20°C. Cells were stained for 1 hour with ab16879 at a 1/100 dilution, washed and then stained with Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed, ab98794 at a 1/200 dilution.
This product has been referenced in:
- Burkard M et al. Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor). Cell Biol Toxicol N/A:N/A (2019). Read more (PubMed: 30627956) »
- Kittipassorn T et al. Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1. Exp Eye Res 181:127-135 (2019). Read more (PubMed: 30677389) »