Anti-Superoxide Dismutase 1抗体(ab16831)


  • 产品名称
    Anti-Superoxide Dismutase 1抗体
    参阅全部 Superoxide Dismutase 1 一抗
  • 描述
    兔多克隆抗体to Superoxide Dismutase 1
  • 宿主
  • 特异性
    There is no cross-reactivity with SOD2,3 and 4 by WB.
  • 经测试应用
    适用于: WB, ICC/IF, IHC-P, IP, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Macaque monkey
  • 免疫原

    Recombinant full length protein corresponding to Human Superoxide Dismutase 1.
    Database link: P00441

  • 阳性对照
    • WB: Mouse brain and mouse liver tissue lysates, HeLa, 293T and Jurkat cell lysates. IHC-P: Human placenta tissue.



Our Abpromise guarantee covers the use of ab16831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 17 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.


  • 功能
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 疾病相关
    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • 序列相似性
    Belongs to the Cu-Zn superoxide dismutase family.
  • 翻译后修饰
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • 细胞定位
    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALS antibody
    • ALS1 antibody
    • Amyotrophic lateral sclerosis 1 adult antibody
    • Cu/Zn SOD antibody
    • Cu/Zn superoxide dismutase antibody
    • Epididymis secretory protein Li 44 antibody
    • HEL S 44 antibody
    • Homodimer antibody
    • hSod1 antibody
    • Indophenoloxidase A antibody
    • IPOA antibody
    • Mn superoxide dismutase antibody
    • SOD antibody
    • SOD soluble antibody
    • SOD1 antibody
    • SOD2 antibody
    • SODC antibody
    • SODC_HUMAN antibody
    • Superoxide dismutase [Cu-Zn] antibody
    • Superoxide dismutase 1 antibody
    • Superoxide dismutase 1 soluble antibody
    • Superoxide dismutase Cu Zn antibody
    • Superoxide dismutase cystolic antibody
    see all


  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : Jurkat cell lysate

    Predicted band size: 17 kDa

  • ab16831 staining human normal placenta tissue. Staining is localised to cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ab16831 staining Superoxide Dismutase 1 in rat bone marrow cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 2% BSA for 2 hours at 25°C. Samples were incubated with the primary antibody (1/250 in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. DAPI used to stain the nucleus.

    See Abreview

  • ab16831 staining superoxide dismutase in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 10 minutes at 21°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary.

    See Abreview

  • All lanes : Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilution

    Lane 1 : 40ug mouse liver homogenate
    Lane 2 : 20ug mouse liver homogenate
    Lane 3 : 5ug mouse liver homogenate

    All lanes : HRP conjugated donkey anti-rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

    Exposure time: 20 seconds

    This image is courtesy of an Abreview submitted by Sandra Sobocanec on 16 March 2006.

    See Abreview

  • Anti-Superoxide Dismutase 1 antibody (ab16831) at 1/2000 dilution + Recombinant human Superoxide Dismutase 1 protein (ab90040) at 0.1 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa

    Exposure time: 4 minutes


This product has been referenced in:
  • Capper MJ  et al. The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation. Nat Commun 9:1693 (2018). Read more (PubMed: 29703933) »
  • Bodega G  et al. The Antioxidant Machinery of Young and Senescent Human Umbilical Vein Endothelial Cells and Their Microvesicles. Oxid Med Cell Longev 2017:7094781 (2017). WB ; Human . Read more (PubMed: 28642812) »

See all 46 Publications for this product


Vielen Dank für Ihre Antwort.

Ich kann bestätigen, dass die Immunogensequenz von ab52950die Mutation G93A nicht beinhaltet und somit beide Proteine erkennen wird. (Ich nehme hierbei an, dass G93A eine Punktmutation ist, die nicht zu...

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Thank you for your patience in this matter. I have received a reply from the original of ab16831 (Superoxide Dismutase 1 antibody). By WB, this product shows no cross-reactivity with SOD2, SOD3, or SOD4. This information has been added to the prod...

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