Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293FT cell line)
Specification
293FT cell line
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit IgG Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
The 293 FT cells were cultured for 3 days and fixed in 3.7% formaldehyde and blocked with 5% BSA in PBS for 1 hr. Then the cells were incubated with the primary antibody overnight at +4°C.
The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG and the nucleus were stained with DAPI.
The cytoplasm of cell(right side)had little bit shrunk but when merged with DAPI, SOD1 observed in the cytoplasm.
The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG and the nucleus were stained with DAPI.
The cytoplasm of cell(right side)had little bit shrunk but when merged with DAPI, SOD1 observed in the cytoplasm.
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提交于 Jan 22 2010