Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
1 µg
Specification
HeLa cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3%BSA in PBST buffer
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti rabbit IgG DyLight 680
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 680
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 680
Detection
Detection method
Odyssey imaging system
Exposure
10 second(s)
Bands
Specific: 18 kDa
Additional data
Additional Notes
Free sumo2/3 (~18kDa) and conjugated sumo2/3 at higher MW are observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 29 2017