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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-STUB1/CHIP抗体(ab2917)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (11)

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Western blot - Anti-STUB1/CHIP antibody (ab2917)
  • Western blot - Anti-STUB1/CHIP antibody (ab2917)
  • Western blot - Anti-STUB1/CHIP antibody (ab2917)

Key features and details

  • Rabbit polyclonal to STUB1/CHIP
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Rabbit, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-STUB1/CHIP抗体
    参阅全部 STUB1/CHIP 一抗
  • 描述

    兔多克隆抗体to STUB1/CHIP
  • 宿主

    Rabbit
  • 特异性

    Detects human carboxyl terminus of hsc70-interacting protein (CHIP).Detects a band of approximately 35 kDa representing CHIP from COS-1 cells overexpressing the human gene including a non-specific band at 80kDa . A customer reported that in mouse cerebrum the antibody detects a band of 35kDa as well as two small mon-specific at 44 and 70 kDa.
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Rabbit, Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide corresponding to Human STUB1/CHIP aa 218-232.
    Sequence:

    VDEKRKKRDIPDYLC


    (Peptide available as ab4934)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Previously labelled as STUB1.  

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    A recently identified protein, termed carboxyl terminus of hsc70-interacting protein (CHIP), has been shown to interact both with the constitutive form of hsc70 and the stress inducible form, hsp70. This novel 35 kDa cytoplasmic protein has been shown to be highly expressed in striated muscle in vivo. Additional studies have shown that this protein is expressed over a broad range of cultured tissues. Through immunoprecipitation experiments, CHIP has been shown to directly bind to the carboxyl terminus of hsc70 and hsp70 where it decreases ATPase activity and reduces overall chaperone efficiency. CHIP has also been identified as an important protein in the ubiquitin-proteasome system. CHIP contains a U-box domain and acts as an E3 ubiquitin-ligase in conjunction with hsc70 and hsp90.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • SMADs
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human STUB1/CHIP protein (ab82791)
  • Recombinant Protein

    • Recombinant Human STUB1/CHIP protein (His tag) (ab206161)

应用

Our Abpromise guarantee covers the use of ab2917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.

靶标

  • 功能

    E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
  • 组织特异性

    Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
  • 通路

    Protein modification; protein ubiquitination.
  • 序列相似性

    Contains 3 TPR repeats.
    Contains 1 U-box domain.
  • 结构域

    The TPR domain is essential for ubiquitination mediated by UBE2D1.
  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession Q9UNE7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 426918 Chicken
    • Entrez Gene: 10273 Human
    • Entrez Gene: 56424 Mouse
    • Entrez Gene: 287155 Rat
    • Omim: 607207 Human
    • SwissProt: Q5ZHY5 Chicken
    • SwissProt: Q9UNE7 Human
    • SwissProt: Q9WUD1 Mouse
    • Unigene: 592081 Human
    • Unigene: 491120 Mouse
    • Unigene: 6504 Rat
    see all
  • 别名

    • Antigen NY CO 7 antibody
    • Antigen NY-CO-7 antibody
    • C terminus of Hsp70-interacting protein antibody
    • Carboxy terminus of Hsp70 interacting protein antibody
    • Carboxy terminus of Hsp70-interacting protein antibody
    • Carboxy terminus of Hsp70p interacting protein antibody
    • CHIP antibody
    • CHIP_HUMAN antibody
    • CLL associated antigen KW 8 antibody
    • CLL-associated antigen KW-8 antibody
    • E3 ubiquitin protein ligase CHIP antibody
    • E3 ubiquitin-protein ligase CHIP antibody
    • Heat shock protein A binding protein 2 (c terminal) antibody
    • HSPABP2 antibody
    • NY CO 7 antibody
    • PP1131 antibody
    • SDCCAG7 antibody
    • Serologically defined colon cancer antigen 7 antibody
    • STIP1 homology and U Box containing protein 1 antibody
    • STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase antibody
    • STIP1 homology and U box-containing protein 1 antibody
    • STUB 1 antibody
    • STUB1 antibody
    • UBOX 1 antibody
    • UBOX1 antibody
    see all

图片

  • Western blot - Anti-STUB1/CHIP antibody (ab2917)
    Western blot - Anti-STUB1/CHIP antibody (ab2917)

    Western blot detection of transfected COS-1 cells expressing STUB1/CHIP using ab2917.

  • Western blot - Anti-STUB1/CHIP antibody (ab2917)
    Western blot - Anti-STUB1/CHIP antibody (ab2917)

    Western blot analysis of STUB1/CHIP was performed by loading 20ug of total protein extracted from the left hemisphere (Left lane)or right hemisphere (right lane) of a normal C57BL/6 mouse brain per well on an SDS-PAGE gel. Proteins were transferred to a membrane, blocked with 5% non-fat dry milkand probed with a STUB1/CHIP polyclonal antibody (ab2915) at a dilution of 1:1000, followed by a HRP-conjugated goat anti-rabbit IgG secondary antibody. Detection was performed using a chemiluminescent substrate.

  • Western blot - Anti-STUB1/CHIP antibody (ab2917)
    Western blot - Anti-STUB1/CHIP antibody (ab2917)

实验方案

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (11)

    发表研究结果有使用 ab2917?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2917 被引用在 11 文献中.

    • Mol MO  et al. Clinical and pathologic phenotype of a large family with heterozygous STUB1 mutation. Neurol Genet 6:e417 (2020). PubMed: 32337344
    • Blasco-Benito S  et al. Therapeutic targeting of HER2-CB2R heteromers in HER2-positive breast cancer. Proc Natl Acad Sci U S A 116:3863-3872 (2019). PubMed: 30733293
    • Cvjetkovic A  et al. Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation. Sci Rep 6:36338 (2016). WB . PubMed: 27821849
    • Shi C  et al. The Inhibition of Heat Shock Protein 90 Facilitates the Degradation of Poly-Alanine Expanded Poly (A) Binding Protein Nuclear 1 via the Carboxyl Terminus of Heat Shock Protein 70-Interacting Protein. PLoS One 10:e0138936 (2015). Mouse . PubMed: 26414348
    • Sisoula C  et al. CHIP-dependent p53 regulation occurs specifically during cellular senescence. Free Radic Biol Med 50:157-65 (2011). WB, ICC/IF ; Human . PubMed: 20974249
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-STUB1 / CHIP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 09 2017

    Western blot abreview for Anti-STUB1/CHIP antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Loading amount
    30 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 19 2006

    Question

    What is the concentration for ab2917, lot# 63868.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 15 2004

    Answer

    Ab2917, lot 63868 has a concentration of 1 mg/ml. Have a nice day.

    Read More

    Abcam Scientific Support

    回复于 Dec 15 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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