Key features and details
- Goat polyclonal to STUB1/CHIP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Dog, Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab129295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-P||Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
功能E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4.
组织特异性Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.
通路Protein modification; protein ubiquitination.
序列相似性Contains 3 TPR repeats.
Contains 1 U-box domain.
结构域The TPR domain is essential for ubiquitination mediated by UBE2D1.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.
- Information by UniProt
- Antigen NY CO 7 antibody
- Antigen NY-CO-7 antibody
- C terminus of Hsp70-interacting protein antibody
ab129295, at 3.75µg/ml, staining STUB1/CHIP in Formalin-fixed, Paraffin-embedded Human small intestine (myenteric plexus) tissue by Immunohistochemistry.
Anti-STUB1/CHIP antibody (ab129295) at 0.3 µg/ml + Human muscle lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Primary ab129295 incubated for one hour