Streptavidin HRP (ready-to-use) (ab64269)
概述
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产品名称
Streptavidin HRP (ready-to-use) -
经测试应用
适用于: IHC-Pmore details -
常规说明
Streptavidin-HRP for use with biotinylated secondary antibodies during IHC / immunohistochemistry. Optimized, ready-to-use format.
This product was previously called Streptavidin-Peroxidase.
IHC protocol suitable for use with Streptavidin-HRP ab64269:
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in antibody diluent and incubate.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP ab64269 and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved. Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
性能
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形式
Liquid -
存放说明
Store at +4°C. -
存储溶液
pH: 7.60
Preservative: 0.2% Proclin 300
Constituent: PBS -
Concentration information loading... -
研究领域
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相关性
Streptavidin peroxidase is used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin Peroxidase (Ready to Use) (ab64269)ab64269 Straptavidin Peroxidase used along with an anti-CD20 antibody on Human Tonsil
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin Peroxidase (Ready to Use) (ab64269)Human colon carcinoma stained with anti-p53 antibody using Streptavidin Peroxidase (ab64269).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab64269 被引用在 9 文献中.
- Matouskova K et al. Exposure to low doses of oxybenzone during perinatal development alters mammary gland morphology in male and female mice. Reprod Toxicol 92:66-77 (2020). PubMed: 31408669
- Santini MP et al. Tissue-Resident PDGFRa+ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner. Cell Rep 30:555-570.e7 (2020). PubMed: 31940496
- Kolla S et al. Bisphenol S alters development of the male mouse mammary gland and sensitizes it to a peripubertal estrogen challenge. Toxicology 424:152234 (2019). PubMed: 31201878
- Petrovic R et al. Mouse strain and sex as determinants of immune response to trivalent influenza vaccine. Life Sci N/A:N/A (2018). PubMed: 29859986
- Pokharel A et al. Asymmetric development of the male mouse mammary gland and its response to a prenatal or postnatal estrogen challenge. Reprod Toxicol 82:63-71 (2018). PubMed: 30315872
- LaPlante CD & Vandenberg LN Data describing lack of effects of 17a-ethinyl estradiol on mammary gland morphology in female mice exposed during pregnancy and lactation. Data Brief 14:337-343 (2017). PubMed: 28795111
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). PubMed: 28825717
- Wilson R et al. Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing. PLoS One 9:e100572 (2014). PubMed: 24963654
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). PubMed: 21371998