Application
Western blot
I performed western blot with biotinylated proteins (BioID-tagged) transfected into HEK293T cells. The results clearly show a band at the expected molecular weight. Streptavidin (HRP) was used at 1:500 dilution in TBST with 2.5% BSA and incubated with the membrane 2 hours at room temperature.
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提交于 Aug 07 2020