Detailed protocol was described below.
*5 uL of human serum was separated with size exclusion chromatography into 10 fractions.
*1st antibody : anti-CD9 (Ancell Corporation)
*Blocking : 5% BSA in PBS
*2nd antibody : Biotinylated anti-CD9 (Ancell Corporation)
*HRP label : ab7403 - Streptavidin HRP, 1/30,000 dilution in 1% BSA/PBS, 30 min, RT
*1-Step Ultra TMB-ELISA Substrate Solution - Pierce
*Measure OD 450 nm
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提交于 Aug 20 2014