重组Anti-STRAD抗体[EPR15603] (ab192879)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15603] to STRAD
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-STRAD抗体[EPR15603]
参阅全部 STRAD 一抗 -
描述
兔单克隆抗体[EPR15603] to STRAD -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- 293, HepG2, HeLa and Human fetal brain lysates; Human kidney and rat cardiac muscle tissues; A549 cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15603 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab192879于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
Purified format. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/400.
|
说明 |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. Purified format. |
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/400. |
靶标
-
相关性
Members of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1. -
细胞定位
Cytoplasmic and Nuclear -
数据库链接
- Entrez Gene: 92335 Human
- Entrez Gene: 72149 Mouse
- Entrez Gene: 303605 Rat
- Omim: 608626 Human
- SwissProt: Q7RTN6 Human
- SwissProt: Q3UUJ4 Mouse
- SwissProt: Q7TNZ6 Rat
-
别名
- Protein kinase LYK5 antibody
- Serologically defined breast cancer antigen NY BR 96 antibody
- STE20 related adapter protein antibody
see all
图片
-
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling STRAD antibody (red) with purified ab192879 at a dilution of 1/200. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
-
All lanes : Anti-STRAD antibody [EPR15603] (ab192879) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling STRAD with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling STRAD with ab192879 at a 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG as a secondary antibody. Counter stained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% triton X-100 permeabilized A549 cells labeling STRAD with ab192879 at a 1/400 dilution, followed by Goat anti rabbit IgG(Alexa Fluor® 488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain: Dapi (blue).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (2)
ab192879 被引用在 2 文献中.
- Gao L et al. Kinsenoside Alleviates Alcoholic Liver Injury by Reducing Oxidative Stress, Inhibiting Endoplasmic Reticulum Stress, and Regulating AMPK-Dependent Autophagy. Front Pharmacol 12:747325 (2021). PubMed: 35115920
- Zeng J et al. PrLZ increases prostate cancer docetaxel resistance by inhibiting LKB1/AMPK-mediated autophagy. Theranostics 8:109-123 (2018). PubMed: 29290796