Anti-STEAP1抗体(ab3679)

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Western blot - Anti-STEAP1 antibody (ab3679)

    Key features and details

    • Rabbit polyclonal to STEAP1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-STEAP1抗体
      参阅全部 STEAP1 一抗

    • 描述

      兔多克隆抗体to STEAP1

    • 宿主

      Rabbit

    • 特异性

      This antibody reacts predominantly with STEAP. Ab3679 can be used as a plasma marker for prostate cancer cells

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide corresponding to Human STEAP1 aa 14-28 conjugated to keyhole limpet haemocyanin.
      Sequence:

      WKMKPRRNLEEDDYL

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • WB: A431 cell lysate

    • 常规说明


      Prostate cancer is the most frequently diagnosed cancer and is the second leading cause of cancer-related deaths in American men. Although the early serum detection of prostate-specific antigen (PSA) plays important roles in early diagnosis, it may not distinguish prostate cancer from benign diseases. Recently, a novel protein, which contains 339 amino acids, named STEAP (six transmembrane epithelial antigen of the prostate), was identified in advanced prostate cancer. STEAP is unique among the currently known prostate cancer markers because of its putative secondary structure, from which one may predict that it functions as a potential channel protein. STEAP is strongly expressed in advanced prostate cancer and some extent expression in other cancers, such as colon and ovarian cancer cell lines.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • Primary antibody说明

      Prostate cancer is the most frequently diagnosed cancer and is the second leading cause of cancer-related deaths in American men. Although the early serum detection of prostate-specific antigen (PSA) plays important roles in early diagnosis, it may not distinguish prostate cancer from benign diseases. Recently, a novel protein, which contains 339 amino acids, named STEAP (six transmembrane epithelial antigen of the prostate), was identified in advanced prostate cancer. STEAP is unique among the currently known prostate cancer markers because of its putative secondary structure, from which one may predict that it functions as a potential channel protein. STEAP is strongly expressed in advanced prostate cancer and some extent expression in other cancers, such as colon and ovarian cancer cell lines.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab3679 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).

    靶标

    • 功能

      Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.

    • 组织特异性

      Ubiquitously expressed. Highly expressed in prostate tumors.

    • 序列相似性

      Belongs to the STEAP family.
      Contains 1 ferric oxidoreductase domain.

    • 细胞定位

      Endosome membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 2410007B19Rik antibody
        • EC 1.16.1.- antibody
        • Metalloreductase STEAP1 antibody
        • MGC19484 antibody
        • PRSS 24 antibody
        • PRSS24 antibody
        • Six transmembrane epithelial antigen of prostate antibody
        • Six transmembrane epithelial antigen of the prostate 1 antibody
        • Six transmembrane epithelial antigen of the prostate antibody
        • Six-transmembrane epithelial antigen of prostate 1 antibody
        • STEA1_HUMAN antibody
        • STEAP 1 antibody
        • Steap antibody
        • STEAP1 antibody
        see all

      图片

      • Western blot - Anti-STEAP1 antibody (ab3679)
        Western blot - Anti-STEAP1 antibody (ab3679)
        Anti-STEAP1 antibody (ab3679) at 2 µg/ml + A431 cell lysate

        Predicted band size: 38 kDa

      文献 (1)

      发表研究结果有使用 ab3679？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3679 被引用在 1 文献中.

      • Jiang JN  et al. EIF4E regulates STEAP1 expression in peritoneal metastasis. J Cancer 11:990-996 (2020). PubMed: 31949502

      客户评价及客户问答

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      Question

      Sorry for the delay, however I just heard back from the customer after several contact attempts. I have attached their answers to your questions. In answer to your questions: 1) We see many non specific bands (please see picture attached for a sample) 2) Antibody is being tested on EL-4 (mouse T cells) that have been transfected with pcDNA3 encoded with human STEAP. WB was done on whole cell extract. 3) Sample were lysed with SDS, protease inhibitors, b-mercapto, EDTA, etc. Samples were heated for 10 min at 100'C. 4) Ab was raised against Human STEAP. Dilutions used were 1/500, 1/1000, 1/2000, 1/6000. Incubation time of 1hour followed by three thorough washing steps. 5) Secondary antibody is an anti-rabbit. Incubated for 45 min followed by three thorough washing steps. Secondary antibody is commonly used by numerous members of our lab with no problems. 6) ECL kit. 7) Secondary antibody has been run alone (with no primary antibody) and shows no background whatsoever. Blocking and washing steps are sufficient and stringent. We block o/n with agitation at 4 degrees. Washing is has been done 3 times for 10 min each, or 6 times for 5 min each. Bands cannot be degraded products as they are at a higher molecular weight then the full-length expected Band size. 8) Westerns were done many times with the same results. Altered steps were washing times, antibody concentration, altered incubation times. 9) Negative controls showed the same result to a lesser intensity.no positive controls were used. Just to recap, this is the customer that found the same result with his transfected and non-transfected cells (control) and saw no difference in staining. He does not get the 40kDa band that he expects to see (I will ask to see if the customer can show the MWts of the control bands), but a lot of other bands instead. Customer is very unhappy and wants a credit. I look forward to your response,

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      Answer

      Thank you for your enquiry and your patience. We are very sorry that the customer is having problem with this product. We have read through the forwarded protocol and it seems to be fine. After careful consideration, we offer a credit note. We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

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