Anti-STAT6抗体(ab227497)
Key features and details
- Rabbit polyclonal to STAT6
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-STAT6抗体
参阅全部 STAT6 一抗 -
描述
兔多克隆抗体to STAT6 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Pig, Rhesus monkey -
免疫原
Recombinant fragment within Human STAT6 (internal sequence). The exact sequence is proprietary.
Database link: P42226 -
阳性对照
- IP: HeLa whole cell lysate. IHC-P: Mouse kidney tissue; C2C12 xenograft tissue. WB: HepG2 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 94 kDa.
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IP |
1/500 - 1/1000.
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IHC-P |
1/100 - 1/1000.
|
说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 94 kDa. |
IP
1/500 - 1/1000. |
IHC-P
1/100 - 1/1000. |
靶标
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功能
Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated following stimulation by IL-4 and IL-3. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 6778 Human
- Entrez Gene: 20852 Mouse
- Entrez Gene: 100153518 Pig
- Omim: 601512 Human
- SwissProt: P42226 Human
- SwissProt: P52633 Mouse
- Unigene: 524518 Human
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别名
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
see all
图片
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Paraffin-embedded mouse kidney tissue stained for STAT6 using ab227497 at 1/500 dilution in immunohistochemical analysis.
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Anti-STAT6 antibody (ab227497) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 94 kDa7.5% SDS-PAGE gel.
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STAT6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg ab227497. Western blot was performed from the immunoprecipitate using ab227497. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell lysate 50 μg.
Lane 2: Control IP in HeLa whole cell lysate with 2 μg of preimmune rabbit IgG.
Lane 3: ab227497 IP in HeLa whole cell lysate.
10% SDS-PAGE gel.
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Paraffin-embedded C2C12 (mouse) xenograft tissue stained for STAT6 using ab227497 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227497 尚未被引用在任何文献中。