重组Anti-STAT6抗体[21HCLC] (ab277765)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [21HCLC] to STAT6
- Suitable for: ChIP, WB
- Reacts with: Human
概述
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产品名称
Anti-STAT6抗体[21HCLC]
参阅全部 STAT6 一抗 -
描述
兔重组multiclonal [21HCLC] to STAT6 -
宿主
Rabbit -
经测试应用
适用于: ChIP, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human STAT6 aa 600-700.
Database link: P42226 -
阳性对照
- ChIP: HeLa cells treated with IFN-gamma. WB: HeLa, A431, A549 whole cell lysate, HeLa cells treated with IFN1a.
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常规说明
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
Recombinant Multiclonal -
克隆编号
21HCLC -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab277765于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIP |
Use 3 µg for µg of chromatin.
3 µg |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 94 kDa.
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说明 |
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ChIP
Use 3 µg for µg of chromatin. 3 µg |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 94 kDa. |
靶标
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功能
Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated following stimulation by IL-4 and IL-3. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 6778 Human
- Omim: 601512 Human
- SwissProt: P42226 Human
- Unigene: 524518 Human
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别名
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
see all
图片
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ChIP- qPCR analysis of STAT6 was performed with 3 μg/mL of ab277765 on sheared chromatin from 2 million HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with IFN-gamma (50 ng/mL) for 1h using the MAGnify Chromatin Immunoprecipitation System. Normal rabbit IgG (3 μg/mL) was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus Real-Time PCR System with primers for the promoter of active AP-1 and COX2 gene, used as positive control targets, and the inactive SAT2 satellite repeat, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
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All lanes : Anti-STAT6 antibody [21HCLC] (ab277765) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG-HRP at 1/5000 dilution
Predicted band size: 94 kDa -
Anti-STAT6 antibody [21HCLC] (ab277765) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with IFN1a (150 ng/mL, 15 min)
Predicted band size: 94 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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