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Signal Transduction Signaling Pathway Nuclear Signaling STATs
使用敲除细胞株进行验证重组RabMAb

重组Anti-STAT5b抗体[EPR16671] (ab178941)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (9)

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Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)
  • ChIP - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Flow Cytometry - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Flow Cytometry - Anti-STAT5b antibody [EPR16671] (ab178941)
  • Anti-STAT5b antibody [EPR16671] (ab178941)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16671] to STAT5b
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt, ChIP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

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概述

  • 产品名称

    Anti-STAT5b抗体[EPR16671]
    参阅全部 STAT5b 一抗
  • 描述

    兔单克隆抗体[EPR16671] to STAT5b
  • 宿主

    Rabbit
  • 特异性

    ab178941 shows no cross reactivity with STAT5a.
  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data
  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: K562, HeLa, Jurkat, Daudi whole cell lysates. C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. Mouse and Rat brain, heart, kidney and spleen lysates. Human fetal heart, kidney and spleen lysates. IHC-P: Rat colon, Mouse spleen and Human spleen tissues. ICC/IF: HeLa cells. IP: K562 whole cell extract. ChIP: T-47D cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR16671
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-STAT5b antibody [EPR16671] (ab199767)
    • Alexa Fluor® 647 Anti-STAT5b antibody [EPR16671] (ab199846)
    • Anti-STAT5b antibody [EPR16671] - BSA and Azide free (ab240211)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human STAT5B knockout HeLa cell line (ab266006)
  • KO cell lysates

    • Human STAT5B knockout HeLa cell lysate (ab257710)
  • KO cell pellets

    • Human STAT5B knockout HeLa cell pellet (ab278846)
  • Recombinant Protein

    • Recombinant Human STAT5b protein (ab182829)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ChIP
Human
Flow Cyt
Human
ICC/IF
Human
IHC-P
Mouse
Rat
Human
IP
Human
WB
Mouse
Human
Recombinant fragment
应用 Ab评论 说明
WB (4)
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/40.
Flow Cyt
1/40.
ChIP
Use at an assay dependent concentration.
说明
WB
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.
IP
1/40.
Flow Cyt
1/40.
ChIP
Use at an assay dependent concentration.

靶标

  • 功能

    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
  • 疾病相关

    Growth hormone insensitivity with immunodeficiency
  • 序列相似性

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰

    Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
  • 细胞定位

    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Target information above from: UniProt accession P51692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6777 Human
    • Entrez Gene: 20851 Mouse
    • Entrez Gene: 25126 Rat
    • Omim: 604260 Human
    • SwissProt: P51692 Human
    • SwissProt: P42232 Mouse
    • SwissProt: P52632 Rat
    • Unigene: 595276 Human
    • Unigene: 34064 Mouse
    • Unigene: 54486 Rat
    see all
  • 别名

    • Signal transducer and activator of transcription 5B antibody
    • STA5B_HUMAN antibody
    • STAT5 antibody
    • Stat5b antibody
    • Transcription factor STAT5B antibody
    see all

图片

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/20000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : STAT5B knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Lanes 1-2: Merged signal (red and green). Green - ab178941 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab178941 Anti-STAT5b antibody [EPR16671] was shown to specifically react with STAT5b in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266006 (knockout cell lysate ab257710) was used. Wild-type and STAT5b knockout samples were subjected to SDS-PAGE. ab178941 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 20000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunoprecipitation of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract using ab178941 at 1/40 dilution. Western blot detection of STAT5b utilised ab178941 at 1/2000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution. The blocking and dilution buffer was 5% NFDM/TBST.

  • ChIP - Anti-STAT5b antibody [EPR16671] (ab178941)
    ChIP - Anti-STAT5b antibody [EPR16671] (ab178941)

    Chromatin was prepared from T-47D (starved overnight) treated with Prolactin(10nM 30min) and T-47D(starved overnight) non-treated cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 μg of chromatin, 5 μg of ab178941 (red), or 5 μg of rabbit normal IgG ab172730 (gray) and 20 μL of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers are from PMID: 15686596.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling STAT5b with ab178941 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). Nuclear and cytoplasm staining is detected. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). 

     

    The negative controls are as follows;

    1. ab178941 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) ar 1/200 dilution.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling STAT5b with ab178941 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus staining on lymphocytes of Human spleen is detected. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-STAT5b antibody [EPR16671] (ab178941)
    Flow Cytometry - Anti-STAT5b antibody [EPR16671] (ab178941)

    ab178941 staining STAT5b in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/40. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/20000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 4 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/5000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse spleen lysates
    Lane 5 : Rat brain lysates
    Lane 6 : Rat heart lysates
    Lane 7 : Rat kidney lysates
    Lane 8 : Rat spleen lysates
    Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    All lanes : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/20000 dilution

    Lane 1 : Human fetal heart lysates
    Lane 2 : Human fetal kidney lysates
    Lane 3 : Human fetal spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Western blot - Anti-STAT5b antibody [EPR16671] (ab178941)
    Lane 1 : Anti-STAT5b antibody [EPR16671] (ab178941) at 1/1000 dilution
    Lane 2 : Anti-STAT5a antibody [E289] (ab32043) at 1/5000 dilution

    All lanes : STAT5a recombinant protein

    Developed using the ECL technique.

    Predicted band size: 90 kDa



    WB showing no cross reactivity with STAT5a.

  • Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunoprecipitation - Anti-STAT5b antibody [EPR16671] (ab178941)

    Cross Immunoprecipitation of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract showing no cross reactivity with STAT5a. Protein captured by anti-STAT5a antibody (ab32042) was detected by the same antibody in WB (image 1) but not by anti-STAT5b, ab178941 (image 2).

     

    For WB detection, ab178941 was used at a 1/2000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at a 1/1000 dilution. The blocking and dilution buffer was 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling STAT5b with ab178941 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus staining on lymphocytes of Mouse spleen is detected. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b antibody [EPR16671] (ab178941)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling STAT5b with ab178941 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus staining on lymphocytes and weak nucleus staining on gland epithelium of colon is detected. The negative control utilised PBS insead of primary antibody and the slide is counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-STAT5b antibody [EPR16671] (ab178941)
    Flow Cytometry - Anti-STAT5b antibody [EPR16671] (ab178941)

    Flow cytometry analysis of 2% paraformaldehyde K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling STAT5b with ab178941 at 1:60 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1:150 dilution. The isotype control is rabbit monoclonal IgG (black line).

  • Anti-STAT5b antibody [EPR16671] (ab178941)
    Anti-STAT5b antibody [EPR16671] (ab178941)

实验方案

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

文献 (9)

发表研究结果有使用 ab178941?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab178941 被引用在 9 文献中.

  • Meyer LK  et al. Glucocorticoids paradoxically facilitate steroid resistance in T cell acute lymphoblastic leukemias and thymocytes. J Clin Invest 130:863-876 (2020). PubMed: 31687977
  • Zhang G  et al. Testosterone propionate activated the Nrf2-ARE pathway in ageing rats and ameliorated the age-related changes in liver. Sci Rep 9:18619 (2019). PubMed: 31819135
  • Joseph A  et al. Transcriptional Regulation of Nos2 via STAT5B Binding to Nos2 Gene Promoter Mediates Nitric Oxide Production: Relevance in ß-Cell Maintenance. Cell Physiol Biochem 52:141-155 (2019). PubMed: 30790510
  • Acres MJ  et al. Signal transducer and activator of transcription 5B deficiency due to a novel missense mutation in the coiled-coil domain. J Allergy Clin Immunol 143:413-416.e4 (2019). PubMed: 30205186
  • Xu YP  et al. Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy. J Clin Invest 130:4316-4331 (2019). PubMed: 31310587
  • Lee KM  et al. Inhibition of STAT5A promotes osteogenesis by DLX5 regulation. Cell Death Dis 9:1136 (2018). PubMed: 30429452
  • Wang W  et al. miR-100 maintains phenotype of tumor-associated macrophages by targeting mTOR to promote tumor metastasis via Stat5a/IL-1ra pathway in mouse breast cancer. Oncogenesis 7:97 (2018). PubMed: 30563983
  • Santarlasci V  et al. Musculin inhibits human T-helper 17 cell response to interleukin 2 by controlling STAT5B activity. Eur J Immunol 47:1427-1442 (2017). PubMed: 28612433
  • Miyado M  et al. Parturition failure in mice lacking Mamld1. Sci Rep 5:14705 (2015). WB ; Mouse . PubMed: 26435405

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1-4 of 4 Abreviews

Western blot abreview for Anti-STAT5b antibody [EPR16671]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (HEPG2)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
HEPG2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Dr. Robert Kupp

Verified customer

提交于 Oct 20 2020

Western blot abreview for Anti-STAT5b antibody [EPR16671]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (prostate cancer, DU-145)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
prostate cancer, DU-145
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

提交于 Mar 14 2019

Western blot abreview for Anti-STAT5b [EPR16671] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
monocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Matija Hedl

Verified customer

提交于 Mar 02 2016

Western blot abreview for Anti-STAT5b [EPR16671] antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (dendritic cells)
Specification
dendritic cells
Treatment
50 ng/ml GM-CSF (6hrs) or LPS 1´g/ml(24hrs)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Dr. A Amrani

Verified customer

提交于 Oct 27 2014

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