重组Anti-STAT5抗体[SP283] - C-terminal (ab227687)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP283] to STAT5 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-STAT5抗体[SP283] - C-terminal
参阅全部 STAT5 一抗 -
描述
兔单克隆抗体[SP283] to STAT5 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human bladder, colon adenocarcinoma, endometrial adenocarcinoma, ovarian adenocarcinoma, bladder transitional cell carcinoma, skin squamous cell carcinoma, cervical squamous cell carcinoma and B-cell lymphoma tissues.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP283 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227687于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100.
Boil tissue section in EDTA buffer (Cat# DEBX-050), pH 8.0 for 10 minfollowed by cooling at room temperature for 20 min. Incubate with ab227687 for 10 minutes at room temperature. |
说明 |
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IHC-P
1/100. Boil tissue section in EDTA buffer (Cat# DEBX-050), pH 8.0 for 10 minfollowed by cooling at room temperature for 20 min. Incubate with ab227687 for 10 minutes at room temperature. |
靶标
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功能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 20851 Mouse
- Entrez Gene: 397340 Pig
- Entrez Gene: 24918 Rat
- Entrez Gene: 25126 Rat
- Omim: 601511 Human
see all -
别名
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
图片
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Formalin-fixed, paraffin-embedded human bladder tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human B-Cell lymphoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for STAT5 using ab227687 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab227687 被引用在 3 文献中.
- Yang D et al. Oxidized ATM promotes breast cancer stem cell enrichment through energy metabolism reprogram-mediated acetyl-CoA accumulation. Cell Death Dis 11:508 (2020). PubMed: 32641713
- Li Y et al. Csf2 Attenuated Sepsis-Induced Acute Kidney Injury by Promoting Alternative Macrophage Transition. Front Immunol 11:1415 (2020). PubMed: 32733471
- Liu HW et al. Enhanced Hsa-miR-181d/p-STAT3 and Hsa-miR-181d/p-STAT5A Ratios Mediate the Anticancer Effect of Garcinol in STAT3/5A-Addicted Glioblastoma. Cancers (Basel) 11:N/A (2019). PubMed: 31783691