Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat anti rabbit Alexa 488 invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Yona Goldshmit
Verified customer
提交于 Nov 29 2018