Western blot abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human normal oesopheal cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
10 nM PMA
Specification
human normal oesopheal cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/20000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: goat anti-rabbit secndary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 80 kDa

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提交于 Aug 27 2015

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