概述

  • 产品名称
    Anti-STAT3 (phospho S727)抗体
    参阅全部 STAT3 一抗
  • 描述
    兔多克隆抗体to STAT3 (phospho S727)
  • 宿主
    Rabbit
  • 特异性
    ab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
  • 经测试应用
    适用于: IHC-FoFr, IHC-P, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, African green monkey
  • 免疫原

    Synthetic peptide corresponding to Human STAT3 (phospho S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
    Database link: P40763
    (Peptide available as ab43618, ab82053)

  • 阳性对照
    • IHC: Human breast carcinoma tissue. WB: HeLa and COS7 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab30647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20950279
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with Recombinant Human STAT3 protein (ab43618).
ELISA 1/10000.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • 组织特异性
    Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 疾病相关
    Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • 序列相似性
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰
    Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • 细胞定位
    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • ADMIO antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
    • Signal transducer and activator of transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

图片

  • All lanes : Anti-STAT3 (phospho S727) antibody (ab30647)

    Lane 1 : HeLa cells extract
    Lane 2 : HeLa cells extract with Synthesized non-phosphopeptide
    Lane 3 : HeLa cells extract with Synthesized phosphopeptide

    Predicted band size: 88 kDa
    Observed band size: 88 kDa

  • ab30647 at 1/50 dilution staining STAT3 (phospho S727) in human breast carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). The left image show staining by ab30647 without specific peptide while right image show staining after blocking with peptide.

  • All lanes : Anti-STAT3 (phospho S727) antibody (ab30647) at 1/500 dilution

    Lane 1 : COS7 cell lysate
    Lane 2 : COS7 cell lysate with phospho peptide

    Predicted band size: 88 kDa

  • PFA-fixed frozen rat liver tissue stained for STAT3 (phospho S727) using ab30647 at 1/1000 dilution in immunohistochemical analysis, followed by Alexa Fluor® 488 donkey anti-rabbit.

    See Abreview

文献

This product has been referenced in:
  • Ruggieri S  et al. Stat3-positive tumor cells contribute to vessels neoformation in primary central nervous system lymphoma. Oncotarget 8:31254-31269 (2017). Read more (PubMed: 28415725) »
  • Li H & Chen C Inhibition of autophagy enhances synergistic effects of Salidroside and anti-tumor agents against colorectal cancer. BMC Complement Altern Med 17:538 (2017). Read more (PubMed: 29246220) »
See all 19 Publications for this product

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Application
Western blot
Loading amount
45 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Lung)
Specification
Lung
Treatment
Basel conditions
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

Abcam user community

Verified customer

提交于 Oct 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 May 11 2012

Answer

Thank you for your inquiry.

I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.

Since you are already using fluorescence in the green and red spectrum, I recommend to choose a color in the yellow spectrum to go with it.

These two secondaries will fit this description:

ab96920 (Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed)

Click here (or use the following: https://www.abcam.com/index.html?datasheet=96920).

ab98499 (Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed)

Click here (or use the following: https://www.abcam.com/index.html?datasheet=98499).

Please make sure that the instrument you are planning to use has the right filter sets and lasers to be able to measure all three colors together.

I wish you good luck with your experiments.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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