Anti-STAT1 (phospho Y701)抗体[M135] (ab29045)
Key features and details
- Mouse monoclonal [M135] to STAT1 (phospho Y701)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-STAT1 (phospho Y701)抗体[M135]
参阅全部 STAT1 一抗 -
描述
小鼠单克隆抗体[M135] to STAT1 (phospho Y701) -
宿主
Mouse -
特异性
The antibody detects 84 and 91 kDa stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Human STAT1 (phospho Y701) conjugated to Keyhole Limpet Haemocyanin (KLH).
-
阳性对照
- human SKOV-3 and MDA-MB-468 cells, ab7909, Jurkat cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
-
纯度
Protein A purified -
纯化说明
Mouse monoclonal antibody purified with protein A chromatography. -
克隆
单克隆 -
克隆编号
M135 -
同种型
IgG1 -
研究领域
相关产品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab29045于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/1000. Predicted molecular weight: 87 kDa.
|
说明 |
---|
WB
1/1000. Predicted molecular weight: 87 kDa. |
靶标
-
功能
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. -
疾病相关
Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
ISGylated. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization. - Information by UniProt
-
数据库链接
- Entrez Gene: 6772 Human
- Entrez Gene: 25124 Rat
- Omim: 600555 Human
- SwissProt: P42224 Human
- Unigene: 642990 Human
-
别名
- Signal transducer and activator of transcription 1 91kD antibody
- CANDF7 antibody
- DKFZp686B04100 antibody
see all
图片
-
Lanes 1-2 & 4 : Anti-STAT1 antibody (ab2415)
Lane 3 : Anti-STAT1 (phospho Y701) antibody [M135] (ab29045)
Lanes 5-6 : Anti-STAT3 antibody (ab5073)
Lanes 7-8 : Anti-STAT5 antibody (ab68465)
Lanes 9-10 : anti-Stat5 (Tyr-694)
All lanes :A-431 whole cell lysate (ab7909)
Predicted band size: 87 kDaWestern blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10).
-
Lanes 1-2 : Anti-Stat1
Lanes 3-4 : Anti-STAT1 (phospho Y701) antibody [M135] (ab29045) at 1/1000 dilution
Lanes 1 & 3 : Jurkat cells
Lanes 2 & 4 : Jurkat cells treated with 1mM pervanadate
Predicted band size: 87 kDa
Observed band size: 84,91 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (57)
ab29045 被引用在 57 文献中.
- Liu Y et al. APLNR Regulates IFN-γ signaling via β-arrestin 1 mediated JAK-STAT1 pathway in melanoma cells. Biochem J 479:385-399 (2022). PubMed: 35084016
- Chen J et al. STAT1/IFIT2 signaling pathway is involved in PD-L1-mediated epithelial-to-mesenchymal transition in human esophageal cancer. Clin Transl Oncol 24:927-940 (2022). PubMed: 35107757
- Dhayal S et al. Temporal regulation of interferon signalling in human EndoC-βH1 cells. J Mol Endocrinol 69:299-313 (2022). PubMed: 35521765
- Gibson AR et al. A genetic screen identifies a protective type III interferon response to Cryptosporidium that requires TLR3 dependent recognition. PLoS Pathog 18:e1010003 (2022). PubMed: 35584177
- Shi H et al. Activation of the JAK1/STAT1 signaling pathway is associated with peroxiredoxin 6 expression levels in human epididymis epithelial cells. Folia Histochem Cytobiol 60:226-236 (2022). PubMed: 35929062