Anti-StAR抗体(ab96637)
Key features and details
- Rabbit polyclonal to StAR
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-StAR抗体
参阅全部 StAR 一抗 -
描述
兔多克隆抗体to StAR -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Cow, Dog, Pig -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96637于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent concentration.
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WB | (3) |
1/100 - 1/10000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/1000.
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说明 |
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ICC
Use at an assay dependent concentration. |
WB
1/100 - 1/10000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/1000. |
靶标
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功能
Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. -
组织特异性
Expressed in gonads, adrenal cortex and kidney. -
通路
Steroid metabolism; cholesterol metabolism. -
疾病相关
Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted. -
序列相似性
Contains 1 START domain. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 6770 Human
- Omim: 600617 Human
- SwissProt: P49675 Human
- SwissProt: Q28996 Pig
- Unigene: 521535 Human
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别名
- Cholesterol trafficker antibody
- Luteinizing hormone induced protein antibody
- mitochondrial antibody
see all
图片
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Anti-StAR antibody (ab96637) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa12% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining StAR protein at cytosol with ab96637 at 1/500.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate labeling StAR protein at mitochondria with ab96637 at 1/500 dilution. Blue: Hoechst 33342 staining.
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All lanes : Anti-StAR antibody (ab96637) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 32 kDa12% SDS-PAGE
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All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from murine adrenal gland
Lane 2 : Whole tissue lysate prepared from rat adrenal gland
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 20 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab96637 被引用在 21 文献中.
- Manna PR et al. Expression and Function of StAR in Cancerous and Non-Cancerous Human and Mouse Breast Tissues: New Insights into Diagnosis and Treatment of Hormone-Sensitive Breast Cancer. Int J Mol Sci 24:N/A (2023). PubMed: 36614200
- Mohr MA et al. Puberty enables oestradiol-induced progesterone synthesis in female mouse hypothalamic astrocytes. J Neuroendocrinol 34:e13082 (2022). PubMed: 35000221
- Gudelska M et al. Chemerin Affects P4 and E2 Synthesis in the Porcine Endometrium during Early Pregnancy. Int J Mol Sci 23:N/A (2022). PubMed: 35055130
- Przygrodzka E et al. PKA and AMPK Signaling Pathways Differentially Regulate Luteal Steroidogenesis. Endocrinology 162:N/A (2021). PubMed: 33502468
- Plewes MR et al. Trafficking of cholesterol from lipid droplets to mitochondria in bovine luteal cells: Acute control of progesterone synthesis. FASEB J 34:10731-10750 (2020). PubMed: 32614098