Application
Western blot
Sample
Fish Cell lysate - whole cell (whole embryo at 32hpf)
Gel Running Conditions
Reduced Denaturing
Loading amount
500 cells
Specification
whole embryo at 32hpf
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk block
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti Rabbit HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 58, 46 kDa
Positive control
HEK
Additional data
Additional Notes
Had specific staining for HEK cells at ~96kDa, despite obtaining nonspecific staining at 75, 58 and 40kDa. For zebrafish whole embryo, also got banding at 58kDa and 46kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Karen Ong
Verified customer
提交于 Apr 13 2017