Anti-SREBP2抗体(ab28482)
Key features and details
- Rabbit polyclonal to SREBP2
- Suitable for: ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SREBP2抗体
参阅全部 SREBP2 一抗 -
描述
兔多克隆抗体to SREBP2 -
宿主
Rabbit -
特异性
ab28482 recognises Sterol regulatory element-binding protein 2 (SREBP 2) -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Guinea pig, Cow, Dog, Pig, Chimpanzee -
免疫原
Synthetic peptide corresponding to Rat SREBP2 aa 876-890.
Sequence:AISWLQGDDAAVRSH
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阳性对照
- ICC/IF: Human HeLa cells, rat PC12 and L6 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28482于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/20 - 1/200.
|
说明 |
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ICC/IF
1/20 - 1/200. |
靶标
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功能
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. -
组织特异性
Ubiquitously expressed in adult and fetal tissues. -
序列相似性
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. -
细胞定位
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
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数据库链接
- Entrez Gene: 507102 Cow
- Entrez Gene: 6721 Human
- Entrez Gene: 396675 Pig
- Entrez Gene: 300095 Rat
- Omim: 600481 Human
- SwissProt: Q12772 Human
- SwissProt: Q3T1I5 Rat
- Unigene: 443258 Human
see all -
别名
- AI608257 antibody
- bHLHd2 antibody
- Class D basic helix-loop-helix protein 2 antibody
see all
图片
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Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of Rat PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of rat L6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
实验方案
数据表及文件
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Datasheet download
文献 (24)
ab28482 被引用在 24 文献中.
- Parente M et al. Brain Cholesterol Biosynthetic Pathway Is Altered in a Preclinical Model of Fragile X Syndrome. Int J Mol Sci 23:N/A (2022). PubMed: 35328827
- Wang S et al. Role of sortilin 1 (SORT1) on fatty acid-mediated cholesterol metabolism in primary calf hepatocytes. J Dairy Sci 105:7773-7786 (2022). PubMed: 35940922
- Wei D et al. Characterization of the promoter region of the bovine IRX3 gene: roles of SREBF2 and PPARG. Physiol Genomics 52:160-167 (2020). PubMed: 32036767
- Xu W et al. L-Theanine affects intestinal mucosal immunity by regulating short-chain fatty acid metabolism under dietary fiber feeding. Food Funct 11:8369-8379 (2020). PubMed: 32935679
- Sun Y et al. Novel Lactobacillus reuteri HI120 Affects Lipid Metabolism in C57BL/6 Obese Mice. Front Vet Sci 7:560241 (2020). PubMed: 33195535