Application
Western blot
Sample
Rabbit Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: rt°C
Other product details
Concentration
1 µg/ml
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST 1X
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse Ig G HRP-linked
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL MILLIPORE
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 125 kDa
Positive control
NONE
Negative control
NONE
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Jan 11 2013