Anti-SRC3抗体[AX15.3] - ChIP Grade (ab2782)
Key features and details
- Mouse monoclonal [AX15.3] to SRC3 - ChIP Grade
- Suitable for: ChIP, WB, ELISA, IHC-P, IP
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-SRC3抗体[AX15.3] - ChIP Grade
参阅全部 SRC3 一抗 -
描述
小鼠单克隆抗体[AX15.3] to SRC3 - ChIP Grade -
宿主
Mouse -
经测试应用
适用于: ChIP, WB, ELISA, IHC-P, IPmore details -
种属反应性
与反应: Human -
免疫原
Fusion protein corresponding to Human SRC3 aa 605-1294.
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常规说明
Locus Link: 8202. Alternative names: ACTR, AIB1, RAC3, CTG26, p/CIP, CAGH16, TNRC14, TNRC16, TRAM-1. Alias: Amplified in breast cancer-1.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
AX15.3 -
同种型
IgG1 -
研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab2782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa). | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/10 - 1/100. | |
| IP | Use at an assay dependent concentration. |
靶标
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功能
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9. -
组织特异性
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney. -
序列相似性
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
结构域
Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain). -
翻译后修饰
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function. -
细胞定位
Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8202 Human
- Omim: 601937 Human
- SwissProt: Q9Y6Q9 Human
- Unigene: 592142 Human
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别名
- ACTR antibody
- AIB 1 antibody
- AIB-1 antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)](https://www.abcam.cn/ps/products/2/ab2782/Images/ab2782-219026-anti-src3-antibody-ax153-chip-grade-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human pancreas tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2782 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting
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![Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)](https://www.abcam.cn/ps/products/2/ab2782/Images/ab2782_1.jpg)
Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)Western blot of AIB1 on MCF-7 cell extract using ab2782. Western blot of AIB1 on MCF-7 cell extract using ab2782.
实验方案
数据表及文件
文献 (4)
ab2782 被引用在 4 文献中.
- Wagner M et al. NCOA3 is a selective co-activator of estrogen receptor a-mediated transactivation of PLAC1 in MCF-7 breast cancer cells. BMC Cancer 13:570 (2013). ChIP ; Human . PubMed: 24304549
- Levy N et al. Differential regulation of native estrogen receptor-regulatory elements by estradiol, tamoxifen, and raloxifene. Mol Endocrinol 22:287-303 (2008). ChIP ; Human . PubMed: 17962382
- Levy N et al. Multiple transcription factor elements collaborate with estrogen receptor alpha to activate an inducible estrogen response element in the NKG2E gene. Endocrinology 148:3449-58 (2007). ChIP ; Human . PubMed: 17395694
- Cvoro A et al. Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression. Mol Cell 21:555-64 (2006). ChIP ; Human . PubMed: 16483936
