Anti-SRC3抗体[AX15.3] - ChIP Grade (ab2782)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
  • Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

Key features and details

  • Mouse monoclonal [AX15.3] to SRC3 - ChIP Grade
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-SRC3抗体[AX15.3] - ChIP Grade
    参阅全部 SRC3 一抗

  • 描述

    小鼠单克隆抗体[AX15.3] to SRC3 - ChIP Grade

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Fusion protein corresponding to Human SRC3 aa 605-1294.

  • 常规说明

    Locus Link: 8202. Alternative names: ACTR, AIB1, RAC3, CTG26, p/CIP, CAGH16, TNRC14, TNRC16, TRAM-1. Alias: Amplified in breast cancer-1.

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    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

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应用

Our Abpromise guarantee covers the use of ab2782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
IHC-P 1/10 - 1/100.

靶标

  • 功能

    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.

  • 组织特异性

    Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

  • 序列相似性

    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.

  • 结构域

    Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

  • 翻译后修饰

    Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
    Methylated by CARM1.
    Phosphorylated by IKK complex. Regulated its function.

  • 细胞定位

    Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ACTR antibody
      • AIB 1 antibody
      • AIB-1 antibody
      • AIB1 antibody
      • Amplified in breast cancer 1 antibody
      • Amplified in breast cancer 1 protein antibody
      • bHLHe42 antibody
      • CAGH 16 antibody
      • CAGH16 antibody
      • CBP interacting protein antibody
      • CBP-interacting protein antibody
      • Class E basic helix-loop-helix protein 42 antibody
      • CTG 26 antibody
      • CTG26 antibody
      • KAT13B / AIB1 antibody
      • MGC141848 antibody
      • NCOA 3 antibody
      • NCoA-3 antibody
      • Ncoa3 antibody
      • NCOA3_HUMAN antibody
      • Nuclear receptor coactivator 3 antibody
      • pCIP antibody
      • RAC 3 antibody
      • RAC-3 antibody
      • RAC3 antibody
      • Receptor associated coactivator 3 antibody
      • Receptor-associated coactivator 3 antibody
      • SRC 3 antibody
      • SRC-3 antibody
      • SRC3 antibody
      • Steroid receptor coactivator protein 3 antibody
      • Thyroid hormone receptor activator molecule 1 antibody
      • TNRC14 antibody
      • TNRC16 antibody
      • TRAM 1 antibody
      • TRAM-1 antibody
      • TRAM1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human pancreas tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2782 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting

    • Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
      Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
      Western blot of AIB1 on MCF-7 cell extract using ab2782. Western blot of AIB1 on MCF-7 cell extract using ab2782.

    文献 (4)

    发表研究结果有使用 ab2782？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2782 被引用在 4 文献中.

    • Wagner M  et al. NCOA3 is a selective co-activator of estrogen receptor a-mediated transactivation of PLAC1 in MCF-7 breast cancer cells. BMC Cancer 13:570 (2013). ChIP ; Human . PubMed: 24304549
    • Levy N  et al. Differential regulation of native estrogen receptor-regulatory elements by estradiol, tamoxifen, and raloxifene. Mol Endocrinol 22:287-303 (2008). ChIP ; Human . PubMed: 17962382
    • Levy N  et al. Multiple transcription factor elements collaborate with estrogen receptor alpha to activate an inducible estrogen response element in the NKG2E gene. Endocrinology 148:3449-58 (2007). ChIP ; Human . PubMed: 17395694
    • Cvoro A  et al. Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression. Mol Cell 21:555-64 (2006). ChIP ; Human . PubMed: 16483936

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    Western blot abreview for Anti-SRC3 antibody [AX15.3] - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (huh-7)
    Loading amount
    20000 cells
    Specification
    huh-7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

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    提交于 Jun 16 2009

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