Anti-SRC3抗体(ab2831)
Key features and details
- Rabbit polyclonal to SRC3
- Suitable for: WB, ChIP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SRC3抗体
参阅全部 SRC3 一抗 -
描述
兔多克隆抗体to SRC3 -
宿主
Rabbit -
特异性
Detects amplified in breast cancer 1 (AIB1). -
经测试应用
适用于: WB, ChIP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human SRC3 aa 3-15.
Sequence:GLGENLDPLASDS
(Peptide available asab4915) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 155 kDa. Can be blocked with AIB1 peptide or reuse.
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| ChIP |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 155 kDa. Can be blocked with AIB1 peptide or reuse. |
|
ChIP
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9. -
组织特异性
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney. -
序列相似性
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
结构域
Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain). -
翻译后修饰
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function. -
细胞定位
Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8202 Human
- Entrez Gene: 398021 Xenopus laevis
- Omim: 601937 Human
- SwissProt: Q9Y6Q9 Human
- SwissProt: O57539 Xenopus laevis
- Unigene: 592142 Human
- Unigene: 268 Xenopus laevis
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别名
- ACTR antibody
- AIB 1 antibody
- AIB-1 antibody
see all
图片
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Western blot - Anti-SRC3 antibody (ab2831)Western blot of Human 2831 in T47D cell lysate using ab2831.
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Immunocytochemistry/ Immunofluorescence - Anti-SRC3 antibody (ab2831)ICC/IF image of ab2831 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2831, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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ChIP - Anti-SRC3 antibody (ab2831)This image is courtesy of Sylvain Daujatab2831 can be used in ChIP. You can expect recruitment of AIB1on the pS2 promoter at some point during transcriptional activation. The amount of DNA precipiated is significant.
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab2831 to AIB1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3
µ l of ab2831 and 2x106 cells were used in each ChIP experiment. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody (ab2831)IHC image of ab2831 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2831, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab2831 被引用在 7 文献中.
- Salazar-Silva R et al. NCOA3 identified as a new candidate to explain autosomal dominant progressive hearing loss. Hum Mol Genet 29:3691-3705 (2021). PubMed: 33326993
- Yao C et al. Thevebioside, the active ingredient of traditional Chinese medicine, promotes ubiquitin-mediated SRC-3 degradation to induce NSCLC cells apoptosis. Cancer Lett 493:167-177 (2020). PubMed: 32829007
- He Z et al. SRC3 Is a Cofactor for ROR?t in Th17 Differentiation but Not Thymocyte Development. J Immunol 202:760-769 (2019). PubMed: 30567733
- You D et al. Acetylation Enhances the Promoting Role of AIB1 in Breast Cancer Cell Proliferation. Mol Cells 39:663-8 (2016). WB . PubMed: 27665502
- Mason CE et al. Location analysis for the estrogen receptor-{alpha} reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res : (2010). ChIP ; Human . PubMed: 20047966
- Fenne IS et al. Recruitment of Coactivator GRIP1 to an Estrogen Receptor Transcription Complex is Regulated by the cAMP-dependent Protein Kinase. Endocrinology : (2008). ChIP ; Human . PubMed: 18499756
- Kawagoe J et al. Mechanism of the divergent effects of estrogen on the cell proliferation of human umbilical endothelial versus aortic smooth muscle cells. Endocrinology 148:6092-9 (2007). IP, WB ; Human . PubMed: 17872375