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Signal Transduction Protein Phosphorylation Tyrosine Kinases Src Family

Anti-Src (phospho Y419)抗体(ab4816)

  • Datasheet
  • SDS
Reviews (5)Q&A (5)References (44)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y418) antibody (ab4816)
  • Western blot - Anti-Src (phospho Y418) antibody (ab4816)

Key features and details

  • Rabbit polyclonal to Src (phospho Y419)
  • Suitable for: IHC-P, IHC-FoFr, WB
  • Reacts with: Mouse, Chicken, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Src (phospho Y419)抗体
    参阅全部 Src 一抗
  • 描述

    兔多克隆抗体to Src (phospho Y419)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, IHC-FoFr, WBmore details
  • 种属反应性

    与反应: Mouse, Chicken, Human
  • 免疫原

    Synthetic peptide corresponding to Src (phospho Y419). The sequence is conserved in mouse, rat, chicken and frog.

  • 常规说明


    Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Src, and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a Src-derived peptide that is phosphorylated at tyrosine 418.
  • Primary antibody说明

    Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418 (tyrosine 414 on frog Src, tyrosine 416 on chicken Src, and tyrosine 424 on mouse Src). This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Src Family
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Src family
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Src protein (ab79635)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4816于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/10 - 1/100.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21901128
WB (2)
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
说明
IHC-P
1/10 - 1/100.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21901128
WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

靶标

  • 功能

    Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰

    Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 细胞定位

    Cell membrane. Mitochondrion inner membrane.
  • Target information above from: UniProt accession P12931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6714 Human
    • Entrez Gene: 20779 Mouse
    • Omim: 190090 Human
    • SwissProt: P00523 Human
    • SwissProt: P12931 Human
    • SwissProt: P05480 Mouse
    • Unigene: 195659 Human
    • 形式

      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • 别名

      • ASV antibody
      • Avian sarcoma virus antibody
      • AW259666 antibody
      • c SRC antibody
      • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
      • cSrc antibody
      • EC 2.7.10.2 antibody
      • Neuronal CSRC tyrosine specific protein kinase antibody
      • Neuronal proto-oncogene tyrosine-protein kinase Src antibody
      • Neuronal SRC antibody
      • Oncogene SRC antibody
      • OTTHUMP00000174476 antibody
      • OTTHUMP00000174477 antibody
      • p60 Src antibody
      • p60-Src antibody
      • p60c-src antibody
      • p60Src antibody
      • pp60c src antibody
      • pp60c-src antibody
      • pp60csrc antibody
      • Proto oncogene tyrosine protein kinase Src antibody
      • Proto-oncogene c-Src antibody
      • Proto-oncogene tyrosine-protein kinase Src antibody
      • Protooncogene SRC antibody
      • Protooncogene SRC Rous sarcoma antibody
      • Src antibody
      • SRC Oncogene antibody
      • SRC proto oncogene non receptor tyrosine kinase antibody
      • SRC_HUMAN antibody
      • SRC1 antibody
      • Tyrosine kinase pp60c src antibody
      • Tyrosine protein kinase SRC 1 antibody
      • Tyrosine protein kinase SRC1 antibody
      • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
      • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
      • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y418) antibody (ab4816)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y418) antibody (ab4816)

      Immunohistochemical analysis of paraffin embedded Human colon carcinoma, labelling Src with ab4816. A negative control without ab4816 is shown on the left. Antigen retrieval performed using 10mM sodium citrate, microwaved for 8-15mins, blocked using 3% H2O2-methanol, then treated with ab4816 followed by an HRP-conjugated secondary antibody. Counterstaining with hematoxylin. 

    • Western blot - Anti-Src (phospho Y418) antibody (ab4816)
      Western blot - Anti-Src (phospho Y418) antibody (ab4816)Dr. Michael Schaller, Dept. of Cell Bio. & Anat., Univ. of North Carolina, Chapel Hill, NC.

      Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].

       

       

       

      Western blot detection of Src phosphorylation in extracts of chick embryo fibroblasts expressing wild-type (lanes 2,4,6) or mutant (lanes 1,3,5) pp60src. Truncation of Src at position 518 eliminated phosphorylation at the negative regulatory site [pY529], while increasing phosphorylation at the catalytic site [pY418].

    实验方案

    • Peptide Competition Experiment
    • Western blotting protocol

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (44)

    发表研究结果有使用 ab4816?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4816 被引用在 44 文献中.

    • Kawakami M  et al. A Novel CDK2/9 Inhibitor CYC065 Causes Anaphase Catastrophe and Represses Proliferation, Tumorigenesis, and Metastasis in Aneuploid Cancers. Mol Cancer Ther 20:477-489 (2021). PubMed: 33277443
    • Bußmann L  et al. Analyzing tyrosine kinase activity in head and neck cancer by functional kinomics: Identification of hyperactivated Src family kinases as prognostic markers and potential targets. Int J Cancer 149:1166-1180 (2021). PubMed: 33890294
    • Lyu C  et al. Targeting Gi/o protein-coupled receptor signaling blocks HER2-induced breast cancer development and enhances HER2-targeted therapy. JCI Insight 6:N/A (2021). PubMed: 34343132
    • Cochrane VA  et al. Leptin modulates pancreatic ß-cell membrane potential through Src kinase-mediated phosphorylation of NMDA receptors. J Biol Chem 295:17281-17297 (2020). PubMed: 33037073
    • Uzair ID  et al. Rapid Estrogen and Progesterone Signaling to Dendritic Spine Formation via Cortactin/Wave1-Arp2/3 Complex. Neuroendocrinology 110:535-551 (2020). PubMed: 31509830
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Src (phospho Y418) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RM-1 - mouse prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    RM-1 - mouse prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Dec 07 2020

    Western blot abreview for Anti-Src (phospho Y418) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LNCaP - prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS PAGE)
    Loading amount
    30 µg
    Specification
    LNCaP - prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Dec 29 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Src (phospho Y418) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Dec 19 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Src (phospho Y418) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP - prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    LNCaP - prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Dec 19 2017

    Western blot abreview for Anti-Src (phospho Y418) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela kyoto beta)
    Loading amount
    15 µg
    Specification
    Hela kyoto beta
    Treatment
    siRNA knock down for 48 h
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 05 2010

    Question

    Dear Tech support

    Attached is a complaint form for ab4816 and it's blot picture.

    Please assist with this matter.

    Thank you for your kind help.

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 20 2012

    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that the anti-Src (phospho Y418) antibody(ab4816) has not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    Having reviewed this case, there are a few further details I'd like to clarify:

    1. What exposure time was used to obtain the image shared with us?

    2. Have the samples used in this blot been used to detect any other targets? Eg. loading controls such as actin or tubulin? Or has pan-Src been measured in the samples?

    3. Has the secondary antibody been used successfully with any other primary antibodies?

    There are a few suggestions I would make in order to help optimize the results from ab4816.

    1. I would suggest introducing 0.1% Tween 20 into all the buffers used (blocking, primary and secondary antibody diluents as well as the wash buffer). This can help to reduce non-specific binding of the membrane.

    2. I would also suggest blocking the membrane at room temperature for ˜1-2 hours then incubate the primary antibody overnight at 4 degrees with aggitation. This can improve the specificity observed with antibodies.

    3. I would also suggest performing a "no primary control" in order to find out how much the secondary antibody is contributing to the non-specificity observed.

    4. It may also be worthwhile to reduce the amount of protein loaded on the gel. I would try 10 and 25 ug/well to see how this may improve the signals observed.

    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Abcam Scientific Support

    回复于 Jul 20 2012

    Question

    will test ab4816 in IHC-Fr

    Read More

    Abcam community

    Verified customer

    Asked on Mar 30 2012

    Answer

    Thank you for your phone call.


    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab4816 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Mar 30 2012

    Question

    what is the concentration for the current lot of this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2008

    Answer

    I just received some good news. I was able to obtain the specific concentration for 2 of our current lots, which are: lot 442382, 0.4 mg/mL lot 502322, 0.5 mg/mL

    Read More

    Abcam Scientific Support

    回复于 Sep 26 2008

    Question

    I guess I should have realized that the peptide sequences are sensitive information. The sequence can be found on NCBI gi:89179499. I also pasted the protein sequence below if that is easier. Thank you for your assistance. 1 mgclfskerr sggsdmgvse ridvsrfqtp qqqtvfhvnn ggnegtisql ngtsdgmmgn 61 grggggggga qeretlvaly pydsradgdl sfqkgdamyl ldhsncdwwy vrhqrtgqtg 121 yvprnfvakq qtieseewya gkiprnraer lvlsshlpkg tflireread trefaltird 181 tddqrnggtv khykikrldh dqgyfittrr tfrslqelvr yysdvpdglc cqltfpaprl 241 aptrpdlshd tqqnweiprn qlhlkrklgd gnfgevwygk wrgivevaik tmkpgtmspe 301 aflqeaqimk qcdhpnlvkl yavctreepf yiiteyming sllqylrtdg stlgiqalvd 361 maaqiangmm yleerklvhr dlaarnvlvg dkisgvpvvk vadfglarkl meediyeart 421 gakfpikwta peaatcgnft vksdvwsygi llyeimtkgq vpypgmhnre vveqvelgyr 481 mpmprgcpeq iyeevllkcw dktpdrrptf dtlyhffddy fvstqpnyap psa

    Read More

    Abcam community

    Verified customer

    Asked on May 26 2006

    Answer

    Thank you for your enquiry. I have performed a sequence alignment of the immunogen sequence and I can tell you that the corresponding C.elegans amino acid sequence has 8 out of 14 amino acid residues in common. However it is most dissimilar in the region immediately surrounding the phosphorylated tyrosine. Based upon this, it is very difficult to predict whether or not the antibody will react with C.elegans Src. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jun 01 2006

    Question

    What is the concentration?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2004

    Answer

    Thank you very much for your patience. The batches of ab4816 and ab4817 that we currently have in stock are at a concentration of 0.25 mg/mL. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Aug 17 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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