Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (hCRC)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris pH9
Permeabilization
Yes - Tween-20 (1%)
Specification
hCRC
Blocking step
BSA/FBS in TBS as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA/FBS in TBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: anti ms HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Just nucelar staining as vendor states. Not really sure if cytoplasm should have also staining as in IF...
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提交于 Jul 14 2020