Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
40 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat Anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: ~62 kDa
Positive control
Starved + Bafilomycin A1
Additional data
Additional Notes
Lane 1: control
Lanes 2 -4: starved in HBSS for 2, 4, and 8 hours, respectively
Lane 5: Starved in HBSS 8 hours + 200 nM Baf A1
Lane 6: Starved in HBSS 8 hours + 4 uM Mg132
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Randal Tibbetts
Verified customer
提交于 Jan 24 2012