Spermidine hydrochloride，nNOS抑制剂(ab120057)
- 数据表
- 参考文献 (1)
概述
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产品名称Spermidine hydrochloride，nNOS抑制剂
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描述Endogenous polyamine. nNOS抑制剂.
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生物学描述Endogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.
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纯度> 98%
性能
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化学名称N-(3-Aminopropyl)butane-1,4-diamine trihydrochloride
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分子量254.63
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化学结构
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分子式C7H19N3.3HCl
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CAS编号334-50-9
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PubChem识别号9539
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存放说明Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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溶解度概述Soluble in water to 100 mM
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处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILESNCCCNCCCCN.Cl.Cl.Cl
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来源
Synthetic
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研究领域
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Nitric oxide & oxidative stress
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
应用
Our Abpromise guarantee covers the use of ab120057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
图片
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Functional Studies - Spermidine hydrochloride (ab120057)ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
文献
This product has been referenced in:
- Baumann P et al. Purification of human Rad51 protein by selective spermidine precipitation. Mutat Res 384:65-72 (1997). Read more (PubMed: 9298115) »
- Hu J et al. Polyamines inhibit nitric oxide synthase in rat cerebellum. Neurosci Lett 175:41-5 (1994). Read more (PubMed: 7526294) »
- Williams K et al. Sensitivity of the N-methyl-D-aspartate receptor to polyamines is controlled by NR2 subunits. Mol Pharmacol 45:803-9 (1994). Read more (PubMed: 8190097) »
