Anti-SP1抗体(ab227383)
Key features and details
- Rabbit polyclonal to SP1
- Suitable for: WB, IP, IHC-P, ChIP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-SP1抗体
参阅全部 SP1 一抗 -
描述
兔多克隆抗体to SP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ChIP, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Rhesus monkey -
免疫原
Recombinant fragment within Human SP1 (internal sequence). The exact sequence is proprietary.
Database link: P08047 -
阳性对照
- WB: HEK-293T and THP-1 whole cell lysate. ICC/IF: HeLa cells. IHC: HeLa and C2C12 xenografts. IP: THP-1 whole cell lysate. ChIP: HEK-293T chromatin extract.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227383于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/20000.
|
|
IP |
1/100 - 1/500.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ChIP |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/100 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/20000. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
ChIP
Use at an assay dependent concentration. |
ICC/IF
1/100 - 1/1000. |
靶标
-
功能
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. -
组织特异性
Up-regulated in adenocarcinomas of the stomach (at protein level). -
序列相似性
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
翻译后修饰
Phosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination, sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation on Ser-7 enhances ubiquitination and protein degradation. Hyperphosphorylation on Ser-101 in response to DNA damage has no effect on transcriptional activity. MAPK1/MAPK3-mediated phosphorylation on Thr-453 and Thr-739 enhances VEGF transcription but, represses FGF2-triggered PDGFR-alpha transcription. Also implicated in the repression of RECK by ERBB2. Hyperphosphorylated on Thr-278 and Thr-739 during mitosis by MAPK8 shielding SP1 from degradation by the ubiquitin-dependent pathway. Phosphorylated in the zinc-finger domain by calmodulin-activated PKCzeta. Phosphorylation on Ser-641 by PKCzeta is critical for TSA-activated LHR gene expression through release of its repressor, p107. Phosphorylation on Thr-668, Ser-670 and Thr-681 is stimulated by angiotensin II via the AT1 receptor inducing increased binding to the PDGF-D promoter. This phosphorylation is increased in injured artey wall. Ser-59 and Thr-681 can both be dephosphorylated by PP2A during cell-cycle interphase. Dephosphorylation on Ser-59 leads to increased chromatin association during interphase and increases the transcriptional activity. On insulin stimulation, sequentially glycosylated and phosphorylated on several C-terminal serine and threonine residues.
Acetylated. Acetylation/deacetylation events affect transcriptional activity. Deacetylation leads to an increase in the expression the 12(s)-lipooxygenase gene though recruitment of p300 to the promoter.
Ubiquitinated. Ubiquitination occurs on the C-terminal proteolytically-cleaved peptide and is triggered by phosphorylation.
Sumoylated by SUMO1. Sumoylation modulates proteolytic cleavage of the N-terminal repressor domain. Sumoylation levels are attenuated during tumorigenesis. Phosphorylation mediates SP1 desumoylation.
Proteolytic cleavage in the N-terminal repressor domain is prevented by sumoylation. The C-terminal cleaved product is susceptible to degradation.
O-glycosylated; contains at least 8 N-acetylglucosamine side chains. Levels are controlled by insulin and the SP1 phosphorylation states. Insulin-mediated O-glycosylation locates SP1 to the nucleus, where it is sequentially deglycosylated and phosphorylated. O-glycosylation affects transcriptional activity through disrupting the interaction with a number of transcription factors including ELF1 and NFYA. Also inhibits interaction with the HIV1 promoter. Inhibited by peroxisomome proliferator receptor gamma (PPARgamma). -
细胞定位
Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location. - Information by UniProt
-
数据库链接
- Entrez Gene: 540741 Cow
- Entrez Gene: 6667 Human
- Entrez Gene: 20683 Mouse
- Entrez Gene: 702710 Rhesus monkey
- Omim: 189906 Human
- SwissProt: P08047 Human
- SwissProt: O89090 Mouse
- Unigene: 620754 Human
see all -
别名
- SP 1 antibody
- SP1 antibody
- Sp1 transcription factor antibody
see all
图片
-
All lanes : Anti-SP1 antibody (ab227383) at 1/10000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : SP1 shRNA transfected HEK-293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions. -
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SP1 (green) using ab227383 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Red: phalloidin, a cytoskeleton marker, diluted at 1/200.
-
ChIP was performed with HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen)chromatin extract and 5 μg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.
-
SP1 was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell lysate with ab227383. Western blot was performed from the immunoprecipitate using ab227383. Anti-rabbit IgG was used as secondary antibody.
Lane 1: THP-1 whole cell lysate (Input).
Lane 2: Rabbit IgG instead of ab227383 in THP-1 whole cell lysate.
Lane 3: ab227383 IP in THP-1 whole cell lysate.
-
Paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) xenograft stained for SP1 using ab227383 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded C2C12 (mouse myoblast cell line) xenograft stained for SP1 using ab227383 at 1/500 dilution in immunohistochemical analysis.
-
Anti-SP1 antibody (ab227383) at 1/2000 dilution + THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.7.5% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (32)
ab227383 被引用在 32 文献中.
- Chen C et al. miRNA-576-5p promotes endometrial cancer cell growth and metastasis by targeting ZBTB4. Clin Transl Oncol 25:706-720 (2023). PubMed: 36538280
- Yang CC et al. Induction of Heme Oxygenase-1 by 15d-Prostaglandin J2 Mediated via a ROS-Dependent Sp1 and AP-1 Cascade Suppresses Lipopolysaccharide-Triggered Interleukin-6 Expression in Mouse Brain Microvascular Endothelial Cells. Antioxidants (Basel) 11:N/A (2022). PubMed: 35453404
- Xu L et al. Hypoxic tumor-derived exosomal circular RNA SETDB1 promotes invasive growth and EMT via the miR-7/Sp1 axis in lung adenocarcinoma. Mol Ther Nucleic Acids 23:1078-1092 (2021). PubMed: 33614250
- Dong W et al. BRG1 Links TLR4 Trans-Activation to LPS-Induced SREBP1a Expression and Liver Injury. Front Cell Dev Biol 9:617073 (2021). PubMed: 33816466
- Kim JS et al. Melatonin activates ABCA1 via the BiP/NRF1 pathway to suppress high-cholesterol-induced apoptosis of mesenchymal stem cells. Stem Cell Res Ther 12:114 (2021). PubMed: 33546749