Anti-SOX8抗体(ab104245)
Key features and details
- Rabbit polyclonal to SOX8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SOX8抗体
参阅全部 SOX8 一抗 -
描述
兔多克隆抗体to SOX8 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Macaque monkey, Gorilla -
免疫原
Synthetic peptide corresponding to Human SOX8 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125935) -
阳性对照
- This antibody gave a positive signal in both Human Testis and Human Colon tissue lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104245于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa). |
靶标
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功能
May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'. -
序列相似性
Contains 1 HMG box DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 30812 Human
- Entrez Gene: 20681 Mouse
- Entrez Gene: 302993 Rat
- Omim: 605923 Human
- SwissProt: P57073 Human
- SwissProt: Q04886 Mouse
- Unigene: 243678 Human
- Unigene: 258220 Mouse
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别名
- MGC24837 antibody
- SOX 8 antibody
- Sox8 antibody
see all
图片
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All lanes : Anti-SOX8 antibody (ab104245) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab104245 被引用在 5 文献中.
- Yuan S et al. SOX8 Affects Tumoral SPARC Expression by Regulating EZH2 to Attenuate Effectiveness of albumin-bound paclitaxel in PDAC. Int J Biol Sci 18:911-922 (2022). PubMed: 35173526
- Du Z et al. SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway. Biomed Res Int 2022:9235837 (2022). PubMed: 36246971
- Katada S et al. Neural stem/precursor cells dynamically change their epigenetic landscape to differentially respond to BMP signaling for fate switching during brain development. Genes Dev 35:1431-1444 (2021). PubMed: 34675062
- Kimura S et al. Sox8 is essential for M cell maturation to accelerate IgA response at the early stage after weaning in mice. J Exp Med 216:831-846 (2019). PubMed: 30877171
- Gryder BE et al. Histone hyperacetylation disrupts core gene regulatory architecture in rhabdomyosarcoma. Nat Genet 51:1714-1722 (2019). PubMed: 31784732