Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Spotted Catshark (Scyliorhinus canicula) Tissue sections (Eye, staining of proliferating cell nuclei)
Specification
Eye, staining of proliferating cell nuclei
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Heat-mediated antigen retrieval by microwave treatment in citric acid working solution pH6
Permeabilization
No
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit immunoglobulin
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200
Additional data
Additional Notes
Dear Abcam,
I would like to use Abcam discount code XXXXXXXX to redeem against a free aliquot of ab29 anti PCNA antibody with my next purchase of an additional aliquot of anti-Sox2 antibody ab97959. This will be purchased during week beginning Monday 25th July 2012 through the University.
I would like to use Abcam discount code XXXXXXXX to redeem against a free aliquot of ab29 anti PCNA antibody with my next purchase of an additional aliquot of anti-Sox2 antibody ab97959. This will be purchased during week beginning Monday 25th July 2012 through the University.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 26 2012