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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Catenins

Anti-SORBS1抗体(ab4551)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Western blot - Anti-SORBS1 antibody (ab4551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab4551)

Key features and details

  • Goat polyclonal to SORBS1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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二抗
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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概述

  • 产品名称

    Anti-SORBS1抗体
    参阅全部 SORBS1 一抗
  • 描述

    山羊多克隆抗体to SORBS1
  • 宿主

    Goat
  • 特异性

    This antibody is expected to recognise both human isoforms.
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide: GTFPGNYVKPLYL, corresponding to C terminal amino acids 804-816 of Human SORBS1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    GenBank Accession Number – NP_006425; NP_056200. LocusLink ID - 10580 (human); 20411 (mouse). Gene Ontology terms - cytoskeletal protein binding; actin binding; cell-matrix adhesion; zonula adherens; cell-cell adherens junction.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4551于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use at an assay dependent concentration.

A 1 hour primary incubation is recommended for this product. No signal obtained yet but low background observed in human heart and 293 lysates at up to 2 µg/ml.

IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration.

A 1 hour primary incubation is recommended for this product. No signal obtained yet but low background observed in human heart and 293 lysates at up to 2 µg/ml.

IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.
  • 组织特异性

    Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle.
  • 序列相似性

    Contains 3 SH3 domains.
    Contains 1 SoHo domain.
  • 翻译后修饰

    O-glycosylated.
  • 细胞定位

    Cell junction > adherens junction. Cell membrane. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation.
  • Target information above from: UniProt accession Q9BX66 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10580 Human
    • Entrez Gene: 20411 Mouse
    • Entrez Gene: 686098 Rat
    • Omim: 605264 Human
    • SwissProt: Q9BX66 Human
    • SwissProt: Q62417 Mouse
    • SwissProt: P84109 Rat
    • Unigene: 38621 Human
    • Unigene: 210815 Mouse
    • Unigene: 417719 Mouse
    • Unigene: 440351 Mouse
    • Unigene: 110441 Rat
    see all
  • 别名

    • c-Cbl-associated protein antibody
    • CAP antibody
    • CBL Associated Protein antibody
    • Ponsin antibody
    • SH3 domain protein 5 antibody
    • SH3D5 antibody
    • SH3P12 antibody
    • Sorbin and SH3 Domain Containing 1 antibody
    • Sorbin and SH3 domain-containing protein 1 antibody
    • Sorbs1 antibody
    • SRBS1_HUMAN antibody
    see all

图片

  • Western blot - Anti-SORBS1 antibody (ab4551)
    Western blot - Anti-SORBS1 antibody (ab4551)
    All lanes : Anti-SORBS1 antibody (ab4551)

    Lane 1 : Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)
    Lane 2 : 10 µl Human plasma 1 (600-800 µg protein)
    Lane 3 : 10 µl Human plasma 2 (600-800 µg protein)
    Lane 4 : 10 µl Human White Blood cells 1 (30-50 µg protein)
    Lane 5 : 10 µl Human White Blood cells 2 (30-50 µg protein)
    Lane 6 : 10 µl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)

    Secondary
    All lanes : Donkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution


    ab4551 was tested in in human plasma, human white blood cells and HEK293 cells. Samples were run for 1h at 200 V using the Fisherbrand vertical complete gel unit, and transferred onto nitrocellulose 0.45um (1h, 100V, 4 C) using the Bio-rad Trans blot cell with plate electrodes. Membranes were blocked for 1 h at RT, then incubated overnight in primary antibody (1/200, 1/1000, 1/5000 or minus primary antibody) at 4 degrees C, with shaking. Following washing, membranes were incubated in secondary antibody (1/5000, Alexa-Fluor680 donkey anti-goat IgG, H+L) for 1 h at RT. Antibody binding was detected using LI-COR Bioscience's Odyssey Infrared Imaging system (intensity 5). The molecular weight of sorbs 1 was calculated using 'Compute pI/MW'from ExPASy tools based on the amino acid sequence according to NP_006425. The band indicated is approximately this predicted molecular weight, and is not present in the minus primary image, scanned at the same intensity.

    Image courtesy of

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab4551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab4551)

    ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows spotty staining on muscle fibres in longitudinal section.

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (2)

发表研究结果有使用 ab4551?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab4551 被引用在 2 文献中.

  • Holle AW  et al. High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation. Integr Biol (Camb) 8:1049-1058 (2016). PubMed: 27723854
  • Hallock PT  et al. Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation. Mol Cell Biol 36:262-70 (2015). IF . PubMed: 26527617

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Western blot abreview for Anti-SORBS1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (white blood cells)
Loading amount
40 µg
Specification
white blood cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Read More

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