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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Catenins

Anti-SORBS1抗体(ab4551)

  • Datasheet
Reviews (1) Submit a question References (2)

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Western blot - Anti-SORBS1 antibody (ab4551)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab4551)

Key features and details

  • Goat polyclonal to SORBS1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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概述

  • 产品名称

    Anti-SORBS1抗体
    参阅全部 SORBS1 一抗
  • 描述

    山羊多克隆抗体to SORBS1
  • 宿主

    Goat
  • 特异性

    This antibody is expected to recognise both human isoforms.
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide: GTFPGNYVKPLYL, corresponding to C terminal amino acids 804-816 of Human SORBS1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    GenBank Accession Number – NP_006425; NP_056200. LocusLink ID - 10580 (human); 20411 (mouse). Gene Ontology terms - cytoskeletal protein binding; actin binding; cell-matrix adhesion; zonula adherens; cell-cell adherens junction.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

Our Abpromise guarantee covers the use of ab4551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

A 1 hour primary incubation is recommended for this product. No signal obtained yet but low background observed in human heart and 293 lysates at up to 2 µg/ml.

IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.
  • 组织特异性

    Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle.
  • 序列相似性

    Contains 3 SH3 domains.
    Contains 1 SoHo domain.
  • 翻译后修饰

    O-glycosylated.
  • 细胞定位

    Cell junction > adherens junction. Cell membrane. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation.
  • Target information above from: UniProt accession Q9BX66 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10580 Human
    • Entrez Gene: 20411 Mouse
    • Entrez Gene: 686098 Rat
    • Omim: 605264 Human
    • SwissProt: Q9BX66 Human
    • SwissProt: Q62417 Mouse
    • SwissProt: P84109 Rat
    • Unigene: 38621 Human
    • Unigene: 210815 Mouse
    • Unigene: 417719 Mouse
    • Unigene: 440351 Mouse
    • Unigene: 110441 Rat
    see all
  • 别名

    • c-Cbl-associated protein antibody
    • CAP antibody
    • CBL Associated Protein antibody
    • Ponsin antibody
    • SH3 domain protein 5 antibody
    • SH3D5 antibody
    • SH3P12 antibody
    • Sorbin and SH3 Domain Containing 1 antibody
    • Sorbin and SH3 domain-containing protein 1 antibody
    • Sorbs1 antibody
    • SRBS1_HUMAN antibody
    see all

图片

  • Western blot - Anti-SORBS1 antibody (ab4551)
    Western blot - Anti-SORBS1 antibody (ab4551)
    All lanes : Anti-SORBS1 antibody (ab4551)

    Lane 1 : Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)
    Lane 2 : 10 µl Human plasma 1 (600-800 µg protein)
    Lane 3 : 10 µl Human plasma 2 (600-800 µg protein)
    Lane 4 : 10 µl Human White Blood cells 1 (30-50 µg protein)
    Lane 5 : 10 µl Human White Blood cells 2 (30-50 µg protein)
    Lane 6 : 10 µl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)

    Secondary
    All lanes : Donkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution


    ab4551 was tested in in human plasma, human white blood cells and HEK293 cells. Samples were run for 1h at 200 V using the Fisherbrand vertical complete gel unit, and transferred onto nitrocellulose 0.45um (1h, 100V, 4 C) using the Bio-rad Trans blot cell with plate electrodes. Membranes were blocked for 1 h at RT, then incubated overnight in primary antibody (1/200, 1/1000, 1/5000 or minus primary antibody) at 4 degrees C, with shaking. Following washing, membranes were incubated in secondary antibody (1/5000, Alexa-Fluor680 donkey anti-goat IgG, H+L) for 1 h at RT. Antibody binding was detected using LI-COR Bioscience's Odyssey Infrared Imaging system (intensity 5). The molecular weight of sorbs 1 was calculated using 'Compute pI/MW'from ExPASy tools based on the amino acid sequence according to NP_006425. The band indicated is approximately this predicted molecular weight, and is not present in the minus primary image, scanned at the same intensity.

    Image courtesy of

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab4551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab4551)

    ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows spotty staining on muscle fibres in longitudinal section.

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (2)

    发表研究结果有使用 ab4551?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4551 被引用在 2 文献中.

    • Holle AW  et al. High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation. Integr Biol (Camb) 8:1049-1058 (2016). PubMed: 27723854
    • Hallock PT  et al. Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation. Mol Cell Biol 36:262-70 (2015). IF . PubMed: 26527617

    客户评价及客户问答

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    Western blot abreview for Anti-SORBS1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (white blood cells)
    Loading amount
    40 µg
    Specification
    white blood cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Read More

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    提交于 Aug 23 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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