重组Alexa Fluor® 405荧光Anti-Sodium Potassium ATPase抗体[EP1845Y] - Plasma膜Loading Control (ab210143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 405 Rabbit monoclonal [EP1845Y] to Sodium Potassium ATPase - Plasma Membrane Loading Control
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Related conjugates and formulations
概述
-
产品名称
Alexa Fluor® 405荧光Anti-Sodium Potassium ATPase抗体[EP1845Y] - Plasma膜Loading Control
参阅全部 Sodium Potassium ATPase 一抗 -
描述
Alexa Fluor® 405荧光兔单克隆抗体[EP1845Y] to Sodium Potassium ATPase - Plasma膜Loading Control -
宿主
Rabbit -
偶联物
Alexa Fluor® 405. Ex: 402nm, Em: 421nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Tilapia -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Flow Cyt (intra)ometry: HeLa cells
-
常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1845Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free (ab167390)
- HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065)
- Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab197713)
- Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)
- Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab198367)
- PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab209299)
- Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab274883)
- APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab314288)
- Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab210143于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/50.
The cellular localisation of this product has been verified in ICC/IF |
说明 |
---|
Flow Cyt (Intra)
1/50. The cellular localisation of this product has been verified in ICC/IF |
靶标
-
功能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
翻译后修饰
Phosphorylation on Tyr-10 modulates pumping activity. -
细胞定位
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
数据库链接
- Entrez Gene: 476 Human
- Entrez Gene: 480 Human
- Entrez Gene: 481 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 11931 Mouse
- Entrez Gene: 27222 Mouse
- Entrez Gene: 24211 Rat
- Entrez Gene: 25650 Rat
see all -
别名
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
图片
-
Overlay histogram showing HeLa cells stained with ab210143 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210143, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Violet laser (405nm) and 450/50 bandpass filter.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab210143 尚未被引用在任何文献中。