Anti-Sodium Potassium ATPase抗体(ab353)
概述
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产品名称Anti-Sodium Potassium ATPase抗体
参阅全部 Sodium Potassium ATPase 一抗 -
描述鸡多克隆抗体to Sodium Potassium ATPase
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宿主Chicken
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特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].
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经测试应用适用于: ICC/IF, IHC-Fr, ELISA, WBmore details
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种属反应性与反应: Dog
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免疫原
ATPase, Sodium, Potassium [Canine Kidney]
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阳性对照
- ICC: MDCK cells ELISA: 1ug recombinant protein
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常规说明Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.878% Sodium chloride -
Concentration information loading...
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纯度Immunogen affinity purified
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纯化说明This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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克隆多克隆
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同种型IgY
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研究领域
相关产品
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Isotype control
应用
Our Abpromise guarantee covers the use of ab353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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ICC/IF | 1/500. (see review). Use amplification. |
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IHC-Fr | 1/100. (see review) |
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ELISA | 1/1000 - 1/6000. This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2’-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. |
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WB | 1/100 - 1/500. |
靶标
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功能This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
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序列相似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
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翻译后修饰Phosphorylation on Tyr-10 modulates pumping activity.
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细胞定位Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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别名
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody (ab353)Image from Qin Y et al, J Cell Biol. 2005 Dec 19;171(6):1061-71. Epub 2005 Dec 12, Fig 7. DOI 10.1083/jcb.200506094ab353 staining Sodium Potassium ATPase in Madin-Darby Canine Kidney (MDCK) cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in methanol/acetone (1:1) at -20°C for 10 minutes, permeabilized with 0.5% Triton X-100 in PBS, and blocked with 5% BSA in PBS for 1 hour before incubation with ab353 at a 1/250 dilution. An Alexa Fluor–conjugated secondary antibody was used at a dilution of 1/500 – 1/1000.
实验方案
数据表及文件
文献
This product has been referenced in:
- Zámbó B et al. Clinically relevant mutations in the ABCG2 transporter uncovered by genetic analysis linked to erythrocyte membrane protein expression. Sci Rep 8:7487 (2018). ICC/IF . Read more (PubMed: 29749379) »
- Martín-Gago P et al. Covalent Protein Labeling at Glutamic Acids. Cell Chem Biol 24:589-597.e5 (2017). Read more (PubMed: 28434875) »