Anti-Sodium Potassium ATPase抗体(ab353)

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Key features and details

  • Chicken polyclonal to Sodium Potassium ATPase
  • Reacts with: Dog
  • Isotype: IgY

概述

  • 产品名称

    Anti-Sodium Potassium ATPase抗体
    参阅全部 Sodium Potassium ATPase 一抗

  • 描述

    鸡多克隆抗体to Sodium Potassium ATPase

  • 宿主

    Chicken

  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].

  • 种属反应性

    与反应: Dog

  • 免疫原

    ATPase, Sodium, Potassium [Canine Kidney]

  • 阳性对照

    • ICC: MDCK cells ELISA: 1ug recombinant protein

  • 常规说明

    Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

相关产品

靶标

  • 功能

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

  • 序列相似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

  • 翻译后修饰

    Phosphorylation on Tyr-10 modulates pumping activity.

  • 细胞定位

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 别名

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody (ab353)
    Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase antibody (ab353)Image from Qin Y et al, J Cell Biol. 2005 Dec 19;171(6):1061-71. Epub 2005 Dec 12, Fig 7. DOI 10.1083/jcb.200506094
    ab353 staining Sodium Potassium ATPase in Madin-Darby Canine Kidney (MDCK) cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol/acetone (1:1) at -20°C for 10 minutes, permeabilized with 0.5% Triton X-100 in PBS, and blocked with 5% BSA in PBS for 1 hour before incubation with ab353 at a 1/250 dilution. An Alexa Fluor–conjugated secondary antibody was used at a dilution of 1/500 – 1/1000.

文献 (9)

发表研究结果有使用 ab353？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab353 被引用在 9 文献中.

  • Zámbó B  et al. Clinically relevant mutations in the ABCG2 transporter uncovered by genetic analysis linked to erythrocyte membrane protein expression. Sci Rep 8:7487 (2018). ICC/IF . PubMed: 29749379
  • Martín-Gago P  et al. Covalent Protein Labeling at Glutamic Acids. Cell Chem Biol 24:589-597.e5 (2017). PubMed: 28434875
  • Braune EB  et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). PubMed: 24449764
  • Liu R  et al. Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking. Autophagy 10:1241-55 (2014). PubMed: 24905460
  • Antalffy G  et al. A C-terminal di-leucine motif controls plasma membrane expression of PMCA4b. Biochim Biophys Acta 1833:2561-72 (2013). ICC/IF . PubMed: 23830917
  • Czepiel M  et al. Differentiation of induced pluripotent stem cells into functional oligodendrocytes. Glia 59:882-92 (2011). PubMed: 21438010
  • Qin Y  et al. The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin. J Cell Biol 171:1061-71 (2005). ICC/IF ; Dog . PubMed: 16344308
  • Tauscher A & Kuver R ABCG5 and ABCG8 are expressed in gallbladder epithelial cells. Biochem Biophys Res Commun 307:1021-8 (2003). IF, IHC ; Dog . PubMed: 12878214
  • Lee J  et al. Polarized cholesterol and phospholipid efflux in cultured gall-bladder epithelial cells: evidence for an ABCA1-mediated pathway. Biochem J 364:475-84 (2002). PubMed: 12023891

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1-4 of 4 Abreviews or Q&A

Question

ab35645: pas de marquage ICC/IF

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Answer

Merci de nous avoir contactés.

Après avoir effectué une recherche pour un anticorps marqueur de la membrane plasmique qui pourrait convenir à vos besoins (autre qu'un anticorps de lapin ou IgG1 de souris),je n'ai trouvé que l'anti-Sodium Potassium ATPase référence https://www.abcam.com/ab353 dont nous avons brièvement parlé au téléphone la semaine dernière.

Cet anticorps polyclonal de poulet (IgY)n'a pour l'instant été testé que chez le chien. Cependant, l'alignement de séquences entre les Sodium/potassium-transporting ATPase subunit alpha-1 humaine et canine montre une homologie de 97% (voirfichier pdf ci-joint). Ceci suppose que l'anticorps ab353 pourrait fonctionner chez l'humain.

Désirez-vous tester cet anticorps en remplacement du ab35645 qui n'a pas fonctionné et pour lequel nous n'avons malheureusement pas d'autre lot que celui que vous avez reçu?

Dans l'attente de votre réponse je vous souhaite une très bonne journée.

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Question

I am interesting in buying an antibody that is specific to Sodium potassium ATPase, that is specific to a peptide of this protein that lies within the cytoplasm. I believe that most commercial ATPase antibodies are specific to regions that are exposed on the cell surface. Do you know whether one of your antibodies actually target the internal face of the plasma membrane?

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Answer

Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from rabbit renal outermedulla and the antibody is a polyclonal antibody the product will recognise a number of epitopes on the protein, some are likely to be at the plasma membrane portion intracellularly and some extracellularly. I hope this information helps, please do not hesitate to contact us again if you need more information,

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Question

The Na pump has 2 subunits, alpha and beta. Which of them is recognized by this ab? How many bands do you see in western blot?

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Answer

This antibody was found to be reactive against the intact enzyme and enzyme subunits (alpha at 100 kDa and beta at 50 kDa) as well as glycosylated and non-glycosylated enzyme forms by western blot. The product is also reactive against 1.0ug of ATPase, Sodium, Potassium in a standard sandwich ELISA.

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Question

Does the ab to canine Na-K-ATPase crossreact with the human or rodent enzyme?

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Answer

Reactivity with other mammalian sources is unknown, but likely, due to the nature of the polyclonal antibody.

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