参阅全部 SOD2/MnSOD 一抗
描述兔单克隆抗体[EPR2560Y] to SOD2/MnSOD
经测试应用适用于: IHC-Fr, WB, IHC-Pmore details
不适用于: Flow Cyt,ICC or IP
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide within Human SOD2/MnSOD aa 1-100 (N terminal). The exact sequence is proprietary.
- WB: Rat brain lysate and SH-SY5Y lysate. IHC-P: Human liver tissue sections.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab68155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21906276|
|WB||1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).|
|IHC-P||1/100 - 1/250.|
功能Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
疾病相关Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
序列相似性Belongs to the iron/manganese superoxide dismutase family.
翻译后修饰Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
- Information by UniProt
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SOD2 knockout HAP1 cell lysate (20 µg)
Lane 3: Human heart tissue lysate (20 µg)
Lane 4: Rat heart tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.
Immunohistochemical analysis of frozen rat lung tissue taken from rats with monocrotaline-exposure/pneumonectomy, staining SOD2 with ab68155.
This product has been referenced in:
- Zhang Z et al. Resolvin D1 promotes corneal epithelial wound healing and restoration of mechanical sensation in diabetic mice. Mol Vis 24:274-285 (2018). Read more (PubMed: 29643724) »
- Zhang J et al. Repeated superovulation increases the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging in mice. Aging (Albany NY) 10:1089-1102 (2018). Read more (PubMed: 29787998) »